Sunila I, Vaccarezza M, Pantaleo G, Fauci A S, Orenstein J M
George Washington University, Medical Center, Department of Pathology, Washington, DC 20037, USA.
Histopathology. 1997 Jan;30(1):31-40. doi: 10.1046/j.1365-2559.1996.d01-559.x.
Persistent generalized lymphadenopathy often develops during HIV-infection. It is characterized by follicular hyperplasia which progresses over time to follicular involution and finally lymphocyte depletion. To determine whether activated cytotoxic T cells (CD8+) are present in the hyperplastic germinal centres, light and electronmicroscopic immunogold labelling with monoclonal antibodies were used to localize two cytotoxic molecules, perforin and TIA-1. Perforin and TIA-1-positive cells were detected in the follicles and paracortex of lymph nodes from HIV-infected patients, whereas labelling was seen only in cells of the paracortex in the hyperplastic lymph nodes from HIV-negative patients. Cytotoxic granules, staining positive for perforin and TIA-1, were identified by transmission electronmicroscopy, often in proximity to follicular dendritic cells within the hyperplastic germinal centres of only HIV-positive patients. These cytotoxic cells may play a role in the follicular dendritic cell loss and concomitant follicular involution that occur during the evolution of HIV disease.
持续性全身性淋巴结病常发生于HIV感染期间。其特征为滤泡增生，随着时间推移发展为滤泡退化，最终淋巴细胞耗竭。为确定增生的生发中心是否存在活化的细胞毒性T细胞（CD8 +），使用单克隆抗体进行光镜和电镜免疫金标记，以定位两种细胞毒性分子，穿孔素和TIA-1。在HIV感染患者的淋巴结滤泡和副皮质中检测到穿孔素和TIA-1阳性细胞，而在HIV阴性患者增生性淋巴结的副皮质细胞中仅见标记。通过透射电子显微镜鉴定出对穿孔素和TIA-1染色呈阳性的细胞毒性颗粒，通常仅在HIV阳性患者增生的生发中心内靠近滤泡树突状细胞处。这些细胞毒性细胞可能在HIV疾病演变过程中发生的滤泡树突状细胞丢失和伴随的滤泡退化中起作用。
