Devergne O, Peuchmaur M, Crevon M C, Trapani J A, Maillot M C, Galanaud P, Emilie D
INSERM U131, Clamart, France.
AIDS. 1991 Sep;5(9):1071-9. doi: 10.1097/00002030-199109000-00002.
Serine esterase B (SE B) is a protein contained in cytoplasmic granules of cytotoxic T lymphocytes and natural killer cells; SE B gene is transcribed upon activation of these cytotoxic cells. In order to show the in vivo interactions between HIV-infected cells and anti-HIV cytotoxic cells we analysed, by in situ hybridization, the expression of the SE B gene in eight hyperplastic lymph nodes from HIV-1-infected patients presenting with persistent generalized lymphadenopathy. We detected numerous cells expressing the SE B gene. The mean number of positive cells was 3.2 times higher in HIV lymph nodes than in six non-HIV hyperplastic lymph nodes studied in parallel (P less than 0.05). In control lymph nodes, the SE B gene was expressed only in interfollicular areas; virtually no cells expressed the SE B gene within follicles. In contrast, in HIV lymph nodes cells expressing the SE B gene were distributed either in interfollicular areas or within follicles. Expression of the SE B gene inside follicles was thus a specific feature of HIV lymph nodes (P less than 0.001) and was associated with the presence of HIV antigens and RNA at the same site. These results suggest that cytotoxic cells are activated in follicles of HIV lymph nodes and may be involved in the lysis of HIV-infected cells. Such a phenomenon may explain the development of follicle lysis, a specific feature of HIV lymph nodes. It may also inhibit the spreading of HIV infection.
丝氨酸酯酶B（SE B）是一种存在于细胞毒性T淋巴细胞和自然杀伤细胞胞质颗粒中的蛋白质；SE B基因在这些细胞毒性细胞被激活时转录。为了展示HIV感染细胞与抗HIV细胞毒性细胞之间的体内相互作用，我们通过原位杂交分析了8例患有持续性全身性淋巴结病的HIV-1感染患者的增生性淋巴结中SE B基因的表达。我们检测到大量表达SE B基因的细胞。HIV淋巴结中阳性细胞的平均数量比同时研究的6个非HIV增生性淋巴结高3.2倍（P小于0.05）。在对照淋巴结中，SE B基因仅在滤泡间区域表达；滤泡内几乎没有细胞表达SE B基因。相反，在HIV淋巴结中，表达SE B基因的细胞分布在滤泡间区域或滤泡内。因此，滤泡内SE B基因的表达是HIV淋巴结的一个特异性特征（P小于0.001），并且与同一部位HIV抗原和RNA的存在相关。这些结果表明，细胞毒性细胞在HIV淋巴结的滤泡中被激活，可能参与HIV感染细胞的裂解。这种现象可能解释了HIV淋巴结的一个特异性特征——滤泡溶解的发生。它也可能抑制HIV感染的传播。
