人血管紧张素原的纯化

Purification of human angiotensinogen.

作者信息

Tewksbury D A, Premeau M R, Dumas M L, Frome W L

出版信息

Circ Res. 1977 Oct;41(4 Suppl 2):29-33. doi: 10.1161/01.res.41.4.29.

DOI:10.1161/01.res.41.4.29

Abstract

A four-step method for the purification of human angiotensinogen has been devised. The four steps are: (1) removal of albumin by affinity chromatography on blue dextran-Sepharose, (2) chromatography on DEAE-Sephadex, (3) chromatography on hydroxylapatite, and (4) chromatography on DEAE-cellulose. This method is capable of producing 8 mg of purified angiotensinogen from 150 ml of plasma with 33% overall recovery of renin-releasable angiotensin I. The angiotensinogen appears homogeneous by immunochemical and ultracentrifugal techniques. The N-terminal amino acids have been determined to be alanine and aspartic acid or asparagine.

摘要

已设计出一种纯化人血管紧张素原的四步法。这四个步骤是：（1）通过在蓝色葡聚糖 - 琼脂糖凝胶上进行亲和层析去除白蛋白；（2）在二乙氨基乙基 - 琼脂糖凝胶上进行层析；（3）在羟基磷灰石上进行层析；（4）在二乙氨基乙基 - 纤维素上进行层析。该方法能够从150毫升血浆中产生8毫克纯化的血管紧张素原，肾素可释放的血管紧张素I的总回收率为33%。通过免疫化学和超速离心技术，血管紧张素原显得均一。已确定其N端氨基酸为丙氨酸和天冬氨酸或天冬酰胺。