Pleten' A P, Mazo V K, Nikulicheva S I, Shaternikov V A
Vopr Med Khim. 1978 Nov-Dec;24(6):798-801.
Angiotensinogen (a substrate of renin) was isolated from pig and sheep blood plasma. Content of angiotensinogen was estimated in the preparations obtained by an indirect biological method, which was based on estimation of an increase in blood pressure in rats, caused by angiotensin, liberated from angiotensinogen during incubation with renin. The preparation, obtained by ammonium sulphate fractionation of pig blood plasma, contained 0.4% of angiotensinogen. Angiotensinogen was also isolated from sheep blood plasma using ammonium sulphate fractionation, DEAE-Sephadex chromatography and gel filtration through Sephadex G-100; this preparation contained 10-13% of angiotensinogen.
血管紧张素原（肾素的一种底物）是从猪和羊的血浆中分离出来的。通过间接生物学方法对所获得的制剂中的血管紧张素原含量进行了估算，该方法基于对与肾素孵育期间从血管紧张素原释放出的血管紧张素所引起的大鼠血压升高的估算。通过硫酸铵分级分离猪血浆获得的制剂含有0.4%的血管紧张素原。血管紧张素原也通过硫酸铵分级分离、DEAE-葡聚糖凝胶色谱法以及通过葡聚糖凝胶G-100进行凝胶过滤从羊血浆中分离出来；该制剂含有10%-13%的血管紧张素原。
