监测抗代谢物疗法中氨甲蝶呤的给药情况。
Monitoring the administration of methotrexate in antimetabolite therapy.
作者信息
Epstein E, Baginski E S, Zac B
出版信息
Ann Clin Lab Sci. 1976 Jul-Aug;6(4):312-7.
PMID:9024
Abstract
An enzymatic method for the measurement of methotrexate (MTX) in serum is presented in which the inhibition of the enzyme dihydrofolate reductase by MTX is measured. Reduction of the substrate dihydrofolate by the enzyme and cofactor NADPH is lessened in direct proportion to the amount of MTX present. Measurements can be made in the "therapeutic range" of MTX which corresponds to the 10(-7) to 10(-8) M concentration of MTX in serum.
摘要
本文介绍了一种用于测量血清中甲氨蝶呤（MTX）的酶法，该方法通过测量MTX对二氢叶酸还原酶的抑制作用来实现。酶和辅因子NADPH对底物二氢叶酸的还原作用会随着MTX含量的增加而直接成比例地减少。MTX的测量范围可涵盖其“治疗范围”，即血清中MTX浓度在10⁻⁷至10⁻⁸ M之间。
相似文献
1
Monitoring the administration of methotrexate in antimetabolite therapy.监测抗代谢物疗法中氨甲蝶呤的给药情况。
Ann Clin Lab Sci. 1976 Jul-Aug;6(4):312-7.
2
High-performance liquid chromatographic and enzyme-inhibition assays of methotrexate concentrations in serum from patients receiving high-dose therapy: tight binding of some methotrexate to a protein that could be dihydrofolate reductase.接受高剂量治疗患者血清中甲氨蝶呤浓度的高效液相色谱法和酶抑制测定：部分甲氨蝶呤与一种可能是二氢叶酸还原酶的蛋白质紧密结合。
Cancer Treat Rep. 1985 Jul-Aug;69(7-8):825-32.
3
A direct ligand-binding radioassay for the measurement of methotrexate in tissues and biological fluids.一种用于测量组织和生物体液中甲氨蝶呤的直接配体结合放射分析方法。
Cancer Res. 1975 Aug;35(8):2033-8.
5
The role of methotrexate and dihydrofolate polyglutamates in the enhancement of fluorouracil action by methotrexate.甲氨蝶呤和二氢叶酸多聚谷氨酸在甲氨蝶呤增强氟尿嘧啶作用中的作用。
Cancer Treat Rep. 1981;65 Suppl 1:29-35.
6
Light activates reduction of methotrexate by NADPH in the ternary complex with Escherichia coli dihydrofolate reductase.在与大肠杆菌二氢叶酸还原酶形成的三元复合物中，光可激活NADPH对甲氨蝶呤的还原作用。
Photochem Photobiol. 1999 Jan;69(1):77-85.
7
A simple radiometric assay for methotrexate and other folate antagonists.一种用于甲氨蝶呤和其他叶酸拮抗剂的简单放射性测定法。
J Lab Clin Med. 1979 Mar;93(3):480-4.
8
Monitoring methotrexate in clinical samples from cancer patients during chemotherapy with a LSPR-based competitive sensor.基于 LSPR 的竞争性传感器监测癌症患者化疗临床样本中的甲氨蝶呤。
Analyst. 2012 Oct 21;137(20):4742-50. doi: 10.1039/c2an35839e.
9
A comparison of dihydrofolate reductase activity in intact leukemia cells and squamous cell carcinoma.完整白血病细胞与鳞状细胞癌中二氢叶酸还原酶活性的比较。
Anticancer Drug Des. 1989 Jun;4(1):79-87.
Zotero 插件