Wilson A S, Stiller R L, Davis P J, Fedel G, Chakravorti S, Israel B A, McGowan F X
Department of Anesthesiology, Children's Hospital of Pittsburgh, PA 15213-2583, USA.
Anesth Analg. 1997 Feb;84(2):315-8. doi: 10.1097/00000539-199702000-00013.
The effects of gestational age (GA) and plasma protein concentrations on the plasma protein binding of fentanyl and alfentanil were studied in preterm and term neonates. Binding experiments were performed using split-cell equilibrium dialysis. Fentanyl and alfentanil concentrations were measured using specific radioimmunoassay, and the proteins albumin and alpha-1-acid glycoprotein (AAG) were measured using radial immunodiffusion assays. In the preterm neonates, 77% of fentanyl and 65% of alfentanil was bound. In the term neonates, 70% of fentanyl and 79% of alfentanil was bound. The binding ratio of alfentanil showed a positive correlation with gestational age and AAG concentration. The binding ratio of fentanyl showed a weak, negative correlation with gestational age. These data indicate that fentanyl and alfentanil are not interchangeable at the GA studied because of age-related changes in protein binding.
在早产儿和足月儿中研究了胎龄（GA）和血浆蛋白浓度对芬太尼和阿芬太尼血浆蛋白结合的影响。使用分室平衡透析进行结合实验。使用特异性放射免疫测定法测量芬太尼和阿芬太尼浓度，使用放射免疫扩散测定法测量蛋白质白蛋白和α-1-酸性糖蛋白（AAG）。在早产儿中，77%的芬太尼和65%的阿芬太尼被结合。在足月儿中，70%的芬太尼和79%的阿芬太尼被结合。阿芬太尼的结合率与胎龄和AAG浓度呈正相关。芬太尼的结合率与胎龄呈弱负相关。这些数据表明，在所研究的胎龄下，由于蛋白质结合的年龄相关变化，芬太尼和阿芬太尼不可互换。
