Googe P B, McGinley K M, Fitzgibbon J F
Department of Pathology, University of Tennessee Medical Center at Knoxville 37920, USA.
Am J Clin Pathol. 1997 Feb;107(2):219-23. doi: 10.1093/ajcp/107.2.219.
Anticytokeratin antibody 34 beta E12 is advocated as an immunohistochemical stain for discriminating benign and malignant lesions of the prostate. Positive staining with 34 beta E12 is said to identify benign lesions, whereas negative staining is said to help substantiate a diagnosis of carcinoma. It is further claimed that 34 beta E12 does not stain prostate carcinoma. The studies leading to these conclusions used hematoxylin-eosin-stained sections of primary prostate lesions as controls. Although the cytokeratin content of a few cell lines of metastatic prostate carcinoma has been investigated, the 34 beta E12 immunohistochemical staining of metastatic prostate carcinoma has not been evaluated. If 34 beta E12 positivity is present only in benign prostate cells, then metastatic prostate carcinoma cells should be uniformly negative with this stain. In 14 cases of moderate and high-grade prostate cancer with metastases to lymph nodes, we found 34 beta E12 positivity in 6 (43%) of 14 metastases and in 7 (54%) of 13 primary tumors. Our findings of 34 beta E12 staining in primary and metastatic moderately and poorly differentiated prostate carcinoma differ from those reported in the literature for well-differentiated prostate carcinoma. We urge caution in the use and interpretation of 34 beta E12 staining for the diagnosis of primary and metastatic prostate carcinoma.
抗细胞角蛋白抗体34βE12被推荐作为一种免疫组织化学染色剂，用于鉴别前列腺的良性和恶性病变。据说34βE12阳性染色可识别良性病变，而阴性染色有助于证实癌的诊断。进一步声称34βE12不会对前列腺癌染色。得出这些结论的研究使用苏木精-伊红染色的原发性前列腺病变切片作为对照。尽管已经研究了少数转移性前列腺癌细胞系的细胞角蛋白含量，但尚未评估转移性前列腺癌的34βE12免疫组织化学染色。如果34βE12阳性仅存在于良性前列腺细胞中，那么转移性前列腺癌细胞用这种染色应该一致呈阴性。在14例发生淋巴结转移的中、高级别前列腺癌病例中，我们发现14个转移灶中有6个（43%）、13个原发性肿瘤中有7个（54%）呈34βE12阳性。我们在原发性和转移性中、低分化前列腺癌中发现的34βE12染色结果与文献中报道的高分化前列腺癌不同。我们敦促在使用和解释34βE12染色用于原发性和转移性前列腺癌的诊断时要谨慎。
