Tanishima K, Minamikawa Y, Yokogawa N, Takeshita M
Clin Chem. 1977 Oct;23(10):1873-7.
We studied the effectiveness of glycerol or ethylene glycol in preventing the increase in alkaline phosphatase activity of lyophilized or refrigerated quality-control serum after reconstitution or repeated freezing and thawing. Control serum reconstituted completely from the lyophilized state with subsequent storage at -20 degrees C showed a considerable decrease in alkaline phosphatase activity immediately after thawing, and a gradual increase in activity on allowing it to stand at room temperature. Adding glycerol or ethylene glycol to the reconstituted serum obviated these changes in activity, glycerol being more effective than ethylene glycol. Reconstituted serum with added glycerol maintained maximum activity before refrigeration during either storage for 30 days or on repeated freezing and thawing. Practical applications of this glycerol-supplemented control serum are discussed.
我们研究了甘油或乙二醇在防止冻干或冷藏的质量控制血清复溶后或反复冻融后碱性磷酸酶活性增加方面的有效性。从冻干状态完全复溶并随后在 -20℃ 储存的对照血清在解冻后立即显示碱性磷酸酶活性显著降低，并且在室温下放置时活性逐渐增加。向复溶的血清中添加甘油或乙二醇可避免活性的这些变化，甘油比乙二醇更有效。添加甘油的复溶血清在冷藏前、储存 30 天期间或反复冻融过程中均保持最大活性。讨论了这种添加甘油的对照血清的实际应用。
