用第三期感染性幼虫对犊牛进行初次感染和连续感染后，对低分子量牛仰口线虫抗原的识别。

Recognition of low molecular weight Cooperia oncophora antigens after primary and trickle infection of calves with third-stage infective larvae.

作者信息

van Diemen P M, Ploeger H W, Nieuwland M G, Rietveld F W, Parmentier H K

机构信息

Department of Animal Husbandry, Agricultural University, Wageningen, The Netherlands.

出版信息

Int J Parasitol. 1996 Nov;26(11):1305-10. doi: 10.1016/s0020-7519(96)00106-3.

DOI:10.1016/s0020-7519(96)00106-3

PMID:9024876

Abstract

Recognition of low molecular protein fragments of adult Cooperia oncophora of calves was studied using sera obtained after a single oral (primary) infection with 100,000 infective 3rd-stage C. oncophora larvae, and after a 10-week period of trickle infection with moderate doses of these larvae. SDS-gel electrophoresis under reducing conditions, followed by western blotting, revealed that a complex of 14-16 kDa protein fragments, a 27 kDa and a 31.6 kDa band of Cooperia oncophora adult antigen were recognized by sera from calves both after primary and trickle infection. A 19 kDa and 30 kDa protein fragment were additionally bound by sera from calves after trickle infection. The data suggest that the systemic humoral immune response of calves to Cooperia oncophora after trickle infection is more pronounced (14-16, 27, 31.6 kDa) and extended (19, 30 kDa) than after primary infection with Cooperia.

摘要

利用单次经口（初次）感染100,000条感染性第三期牛环形泰勒虫幼虫后获得的血清，以及在10周内用中等剂量这些幼虫进行持续感染后获得的血清，研究了犊牛对成年牛环形泰勒虫低分子蛋白片段的识别情况。在还原条件下进行SDS-凝胶电泳，随后进行蛋白质印迹分析，结果显示，初次感染和持续感染后的犊牛血清均能识别牛环形泰勒虫成年抗原的14 - 16 kDa蛋白片段复合物、一条27 kDa和一条31.6 kDa条带。持续感染后的犊牛血清还额外结合了一条19 kDa和一条30 kDa蛋白片段。数据表明，犊牛在持续感染后对牛环形泰勒虫的全身性体液免疫反应比初次感染牛环形泰勒虫后更明显（14 - 16、27、31.6 kDa）且范围更广（19、30 kDa）。