Hirokawa K, Kageyama K, Nakajima T, Tsumura M, Onoyama Y
Department of Radiology, Osaka City University Medical School, Japan.
Int J Hyperthermia. 1997 Jan-Feb;13(1):115-23. doi: 10.3109/02656739709056435.
The change of lactic acid concentration in the tumour after intraperitoneal administration of 5 g/kg glucose, and the effect of the lactic acid concentration on antitumour activity of hyperthermia were studied in an experimental murine tumour. The lactic acid concentration in SCC VII tumours transplanted into the legs of C3H/HeJ male mice was measured by gas chromatography. Local hyperthermic treatment to the tumour was performed with a water bath at 43 degrees C for 40 min. Antitumour effects were evaluated by measuring tumour volume doubling time (DT) as an index. The mean concentration of lactic acid in the tumour was 10.4 mumol/g in the no-treatment group. The lactic acid concentration gradually increased after glucose administration, reaching a significantly high concentration of 20.0 mumol/g at 90 min later. The DTs in the no-treatment group and hyperthermia alone group were 2.4 and 3.7 days respectively. The DTs in the glucose administration groups shortly before, 30 and 60 min before the hyperthermia were 3.6, 3.6 and 5.6 days respectively. The DT in the 60 min group was significantly extended (p < 0.0001). Hyperthermia during the period of increased lactic acid concentration significantly prolonged the DT of the tumour. These results clearly showed that an increase of lactic acid concentration in the tumour improved the effect of local hyperthermia.
在实验性小鼠肿瘤中，研究了腹腔注射5 g/kg葡萄糖后肿瘤中乳酸浓度的变化，以及乳酸浓度对热疗抗肿瘤活性的影响。通过气相色谱法测量移植到C3H/HeJ雄性小鼠腿部的SCC VII肿瘤中的乳酸浓度。用43℃的水浴对肿瘤进行局部热疗40分钟。以测量肿瘤体积倍增时间（DT）作为指标评估抗肿瘤效果。未治疗组肿瘤中乳酸的平均浓度为10.4 μmol/g。给予葡萄糖后，乳酸浓度逐渐升高，90分钟后达到显著高浓度20.0 μmol/g。未治疗组和单纯热疗组的DT分别为2.4天和3.7天。在热疗前不久、热疗前30分钟和60分钟给予葡萄糖的组中的DT分别为3.6天、3.6天和5.6天。60分钟组的DT显著延长（p < 0.0001）。乳酸浓度升高期间的热疗显著延长了肿瘤的DT。这些结果清楚地表明，肿瘤中乳酸浓度的增加提高了局部热疗的效果。
