Dayem M A, Fleury Y, Devilliers G, Chaboisseau E, Girard R, Nicolas P, Delfour A
Laboratoire de Biochimie des Protéines, IBMIG, Université de Poitiers, France.
FEMS Microbiol Lett. 1996 May 1;138(2-3):251-9. doi: 10.1111/j.1574-6968.1996.tb08166.x.
Immunity proteins are though to protect bacteriocin-producing bacterial strains against the bactericidal effects of their own bacteriocin. The immunity protein which protects the lactic acid bacterium Leuconostoc mesenteroides against mesentericin Y105(37) bacteriocin was detected and localized by immunofluorescence and electron microscopy, using antibodies directed against the C-terminal end of the predicted immunity protein. The antibodies recognized the immunity proteins of various strains of Leuconostoc, including Leuconostoc mesenteroides and Leuconostoc gelidum. This study demonstrated that immunity proteins produced by Leuconostoc mesenteroides accumulated in the cytoplasmic compartment of the bacteria. This is in contrast with other known immunity proteins, such as the colicin immunity proteins, which are integral membrane proteins possessing three to four transmembrane domains.
免疫蛋白被认为可以保护产生细菌素的细菌菌株免受自身细菌素的杀菌作用。利用针对预测免疫蛋白C末端的抗体，通过免疫荧光和电子显微镜检测并定位了保护乳酸肠膜明串珠菌免受肠膜菌素Y105(37)细菌素作用的免疫蛋白。这些抗体识别了包括肠膜明串珠菌和嗜冷明串珠菌在内的各种明串珠菌菌株的免疫蛋白。这项研究表明，肠膜明串珠菌产生的免疫蛋白积聚在细菌的细胞质区室中。这与其他已知的免疫蛋白不同，例如大肠杆菌免疫蛋白，它们是具有三到四个跨膜结构域的整合膜蛋白。
