Revol-Junelles A M, Mathis R, Krier F, Fleury Y, Delfour A, Lefebvre G
Laboratoire de Microbiologie Industrielle et Alimentaire, ENSAIA-INPL, Vandoeuvre, France.
Lett Appl Microbiol. 1996 Aug;23(2):120-4. doi: 10.1111/j.1472-765x.1996.tb00045.x.
Mesenterocin 52, a bacteriocin produced by Leuconostoc mesenteroides subsp. mesenteroides FR52, was purified from producing cells by the adsorption-desorption method, combined with reverse-phase high-performance liquid chromatography. The elution profile revealed the presence of two inhibitory peaks of activity, each displaying different inhibitory spectra. Mesenterocin 52A possessed a broad inhibitory spectrum, including anti-Listeria activity, while Mesenterocin 52B was only active against Leuconostoc spp. The amino acid sequence and M(r) of Mesenterocin 52A appeared identical to the previously described Mesentericin Y105. In contrast, Mesenterocin 52B possessed a M(r) of 3446 Da, corresponding to 32 amino acids and a sequence that shared no homology with known bacteriocins: NH2-KGVLGWLSMASSALTGPQQPNSPWLAKIKNHK.
肠膜明串珠菌素52是由肠膜明串珠菌肠膜明串珠亚种FR52产生的一种细菌素，通过吸附-解吸法结合反相高效液相色谱从产生菌细胞中纯化得到。洗脱图谱显示存在两个活性抑制峰，每个峰显示出不同的抑制谱。肠膜明串珠菌素52A具有广泛的抑制谱，包括抗李斯特菌活性，而肠膜明串珠菌素52B仅对明串珠菌属有活性。肠膜明串珠菌素52A的氨基酸序列和相对分子质量与先前描述的肠膜菌素Y105相同。相比之下，肠膜明串珠菌素52B的相对分子质量为3446 Da，对应32个氨基酸，其序列与已知细菌素无同源性：NH2-KGVLGWLSMASSALTGPQQPNSPWLAKIKNHK。
