Kruse K, Bartels H, Günther H
Eur J Pediatr. 1977 Aug 23;126(1-2):53-9. doi: 10.1007/BF00443123.
Using a combination of L-phenylalanine inhibition and heat inactivation, the serum alkaline phosphatase (AP) in 2 to 13 year old children without evidence of hepatobiliary, osseous, or intestinal disease was separated in three fractions; i.e. L-phenylalanine sensitive AP (LPSAP), heat-stable non-L-phenylalanine sensitive AP (heat-stable non-LPSAP) and heat sensitive non-L-phenylalanine sensitive AP (heat-sensitive non-LPSAP). The activities of total AP and the different fractions were measured using optimized test conditions. LPSAP, (mainly intestinal AP), accounts for approximately 12% of the total serum AP activity, heat-stable non-LPSAP (mainly hepatobiliary AP) for approximately 9%, and heat-sensitive non-LPSAP (mainly bone AP) for approximately 77%. To give a better differentiation between bone and liver AP, the percentage ratios of heat-stable non-LPSAP/non-LPSAP (Q value), and heat-stable non-LPSAP/total AP, were determined. Both quotients showed a significant negative correlation with total AP, which has to be taken into account in the interpretation of the results of isoenzyme determinations of serum AP activity. The above semiquantitative separation of AP isoenzymes can be readily done in a routine clinical laboratory.
采用L-苯丙氨酸抑制和热灭活相结合的方法，对2至13岁无肝胆、骨骼或肠道疾病证据的儿童血清碱性磷酸酶（AP）进行了分离，分为三个部分，即L-苯丙氨酸敏感AP（LPSAP）、热稳定的非L-苯丙氨酸敏感AP（热稳定非LPSAP）和热敏感的非L-苯丙氨酸敏感AP（热敏感非LPSAP）。在优化的测试条件下测定总AP和不同部分的活性。LPSAP（主要是肠道AP）约占血清总AP活性的12%，热稳定非LPSAP（主要是肝胆AP）约占9%，热敏感非LPSAP（主要是骨AP）约占77%。为了更好地区分骨AP和肝AP，测定了热稳定非LPSAP/非LPSAP的百分比比值（Q值）以及热稳定非LPSAP/总AP的百分比比值。两个商值均与总AP呈显著负相关，在解释血清AP活性同工酶测定结果时必须考虑这一点。上述AP同工酶的半定量分离在常规临床实验室中很容易完成。
