[已建立的叙利亚仓鼠细胞系的细胞遗传学研究。II. HaK、CER和BHK-21（C-13）细胞系核型的比较分析]
[The cytogenetic study of established Syrian hamster cell lines. II. A comparative analysis of the karyotypes of cell lines HaK, CER and BHK-21 (C-13)].
作者信息
Urmanova M A, Tsareva A A
出版信息
Tsitologiia. 1996;38(6):639-45.
A comparative cytogenetic investigation of the Syrian hamster HaK, CER cell lines and BHK-21 (C-13) sublines was undertaken using G- and C-banding, and silver staining of chromosomes. BHK-21 (C-13) cell sublines were found to have diploid associated chromosome sets, whereas HaK and CER cell lines are characterized with triploid and tetraploid associated chromosome sets, resp. In sum, in all the analysed cell lines 100 marker chromosomes were identified, with 85 ones having unique morphology. The finding indicates a nonaccidental appearance of lesions for normal chromosomes in forming markers. Another arrangement of "hot break-points" on chromosomes is more typical of HaK and CER cell lines, than of BHK-21 (C-13) sublines. The localization of "hot break-points" on chromosomes in BHK-21 (C-13) sublines is specific of the Syrian hamster cells transformed with oncogenic viruses.
利用染色体G显带、C显带和银染技术，对叙利亚仓鼠HaK、CER细胞系以及BHK - 21（C - 13）亚系进行了比较细胞遗传学研究。结果发现，BHK - 21（C - 13）细胞亚系具有二倍体相关染色体组，而HaK和CER细胞系分别具有三倍体和四倍体相关染色体组。总之，在所有分析的细胞系中，共鉴定出100条标记染色体，其中85条具有独特的形态。这一发现表明，在形成标记的过程中，正常染色体出现病变并非偶然。与BHK - 21（C - 13）亚系相比，HaK和CER细胞系中染色体上“热点断点”的另一种排列更为典型。BHK - 21（C - 13）亚系中染色体上“热点断点”的定位是叙利亚仓鼠细胞被致癌病毒转化后的特异性表现。
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