Urmanova M A, Tsareva A A
Tsitologiia. 1996;38(6):646-9.
Using silver staining --> G-banding restaining procedure it was found that the Syrian hamster chromosomes had nucleolar organizers on subtelocentric chromosomes 2, 6, 9, 10 and 13, and on acrocentric chromosomes 16, 17 and 19. In this case, NORs may be recognized only on one of homologous chromosomes, and no more than 9 NO-chromosomes may be found in one metaphase plate. In HaK, BHK-21 (C-13) BKK and BHK-21 (C-13) C cell lines, chromosomes 6, 9, 16, 17 and 19 have NORs, and in each line there are specific marker chromosomes with NOR. The markers consist of the material of chromosomes 6, 9, 16 and 17, with only chromosomes 16 and 17 being incorporated into rearrangements by their NORs. The silver staining --> G-banding restaining procedure makes possible not only identification of NO-chromosomes, but also refinement of the marker origins.
采用银染→G带复染程序发现,叙利亚仓鼠的核仁组织区位于亚中着丝粒染色体2、6、9、10和13以及近端着丝粒染色体16、17和19上。在这种情况下,核仁组织区可能仅在一对同源染色体中的一条上被识别,并且在一个中期板中发现的无核仁组织区染色体不超过9条。在HaK、BHK - 21(C - 13)BKK和BHK - 21(C - 13)C细胞系中,染色体6、9、16、17和19具有核仁组织区,并且在每个细胞系中都有带有核仁组织区的特定标记染色体。这些标记由染色体6、9、16和17的物质组成,只有染色体16和17通过它们的核仁组织区参与重排。银染→G带复染程序不仅能够识别无核仁组织区染色体,还能细化标记的起源。