Piven' N N, Smirnova V I, Viktorov D V, Kovalenko A A, Farber S M, Iarulin R G, Podzolkova G G
Zh Mikrobiol Epidemiol Immunobiol. 1996 Jul-Aug(4):75-8.
Surface antigen 8, one of pathogenicity factors of P.pseudomallei and having an antiphagocytic action, contains glycoprotein with a mol. wt. of 200 kD and proteins with mol wt. of 25, 30 and 34 kD. According to its chemical structure, the carbohydrate part of antigen 8 is the homogeneous polymer of 6-d-D-mannoheptose, and its protein component is a collection of monomer proteins with mol. wt. of 12-120 kD. The consecutive fractionation of antigen 8 by gel and ion exchange chromatography has made it possible to isolate its individual fragments, differing by the collection of antigenic components, as well as by their antiphagocytic and protective activity. Experiments have shown that glycoprotein with a mol. wt. of 200 kD is an active immunosuppressive agent, while protein with a mol. wt. of 34 kD produces a pronounced immunogenic effect.
表面抗原8是类鼻疽伯克霍尔德菌的致病因子之一，具有抗吞噬作用，包含分子量为200 kD的糖蛋白以及分子量为25、30和34 kD的蛋白质。根据其化学结构，抗原8的碳水化合物部分是6 - D -甘露庚糖的均聚物，其蛋白质成分是分子量为12 - 120 kD的单体蛋白的集合。通过凝胶和离子交换色谱对抗原8进行连续分级分离，使得能够分离出其各个片段，这些片段在抗原成分的集合以及抗吞噬和保护活性方面存在差异。实验表明，分子量为200 kD的糖蛋白是一种活性免疫抑制剂，而分子量为34 kD的蛋白质则产生明显的免疫原性效应。
