Knapen M H, Eisenwiener H G, Vermeer C
Cardiovascular Research Institute, University of Limburg, Maastricht, The Netherlands.
Clin Chim Acta. 1996 Dec 30;256(2):151-64. doi: 10.1016/s0009-8981(96)06418-2.
Human serum osteocalcin is a well known bone formation marker. On the basis of their different affinities for hydroxyapatite, the total immunoreactive osteocalcin may be separated into two fractions. Six commercial test kits for osteocalcin were compared. All kits reacted with both osteocalcin fractions but the absolute amounts found in the same serum samples differed widely. During serum storage at room temperature, there was no significant loss of osteocalcin during the first 6 h. After longer storage periods, the recorded decrease of osteocalcin depended on the system used: with two kits, over 80% of the original immunoreactive antigen was left after 9 days. It is considered that the different osteocalcin fractions may become useful as markers for different metabolic bone processes. A more precise definition of the various circulating osteocalcin fractions, and the development of separate tests for each fraction, are requirements for the optimal use of osteocalcin as a diagnostic tool for metabolic bone disorders.
人血清骨钙素是一种众所周知的骨形成标志物。根据它们对羟基磷灰石的不同亲和力，总免疫反应性骨钙素可分为两个部分。对六种市售骨钙素检测试剂盒进行了比较。所有试剂盒都与两种骨钙素部分发生反应，但在相同血清样本中测得的绝对量差异很大。在室温下储存血清期间，最初6小时内骨钙素没有明显损失。储存时间更长后，记录的骨钙素减少情况取决于所使用的系统：使用两种试剂盒时，9天后仍有超过80%的原始免疫反应性抗原留存。据认为，不同的骨钙素部分可能成为不同代谢性骨过程的有用标志物。更精确地定义各种循环骨钙素部分，并为每个部分开发单独的检测方法，是将骨钙素作为代谢性骨疾病诊断工具进行最佳应用的必要条件。
