抗人免疫缺陷病毒1型Nef的鼠单克隆抗体的表位作图

Epitope mapping of murine monoclonal antibodies against human immunodeficiency virus type 1 Nef.

作者信息

Otake K, Fujii Y, Tashiro M, Adachi A, Kitoh J

机构信息

Institute for Laboratory Animal Research, Nagoya University School of Medicine, Japan.

出版信息

Exp Anim. 1997 Jan;46(1):53-8. doi: 10.1538/expanim.46.53.

DOI:10.1538/expanim.46.53

PMID:9027472

Abstract

It has shown that the human immunodeficiency virus type 1 (HIV-1) Nef protein has the high antigenicity in HIV-1 seropostive individuals. We newly obtained seven monoclonal antibodies (mAbs). To identify the antigenic determinants of HIV-1 Nef protein against murine, epitope mapping of the mAbs was performed by enzyme-linked immunosorbent assay (ELISA) by using several recombinant truncated Nef fusion proteins, that were expressed in Esherichia coli, and synthetic peptides. The results showed that mAbs A6, A7, F2, F3, F4, F8 and E5 recognized epitopes on Nef protein located at amino acid residues 18-26, 28-45, 115-137, 128-137, 115-126, 128-137, and 170-181, respectively.

摘要

研究表明，1型人类免疫缺陷病毒（HIV-1）Nef蛋白在HIV-1血清反应阳性个体中具有高抗原性。我们新获得了七种单克隆抗体（mAb）。为了确定HIV-1 Nef蛋白针对鼠类的抗原决定簇，通过酶联免疫吸附测定（ELISA），使用几种在大肠杆菌中表达的重组截短Nef融合蛋白和合成肽，对这些单克隆抗体进行了表位作图。结果显示，单克隆抗体A6、A7、F2、F3、F4、F8和E5分别识别Nef蛋白上位于氨基酸残基18 - 26、28 - 45、115 - 137、128 - 137、115 - 126、128 - 137和170 - 181的表位。