Schneider T, Harthus H P, Hildebrandt P, Niedrig M, Bröker M, Weigelt W, Beck A, Pauli G
AIDS-Zentrum des Bundesgesundheitsamtes, Robert Koch-Institut, Berlin, Federal Republic of Germany.
AIDS Res Hum Retroviruses. 1991 Jan;7(1):37-44. doi: 10.1089/aid.1991.7.37.
Murine monoclonal antibodies (MAbs) raised against a recombinant nef protein fragment of human immunodeficiency virus type 1 (HIV-1) strain BH10 were characterized by an epitope mapping system using overlapping decapeptides. Four different immunogenic regions were identified. Ten human HIV-1-positive sera were tested in the same epitope mapping system, seven of these were reactive with four immunogenic regions. Two of the nef-specific epitopes recognized by human sera overlapped with the epitopes defined by the murine monoclonal antibodies. The reactivity of the monoclonal antibodies with the recombinant nef protein and with infected and uninfected cells were investigated in a variety of test systems. The results are discussed with respect to homologous regions of nef and cellular proteins.
利用重叠十肽的表位作图系统对针对1型人类免疫缺陷病毒（HIV-1）BH10株重组nef蛋白片段产生的鼠单克隆抗体（MAb）进行了特性分析。确定了四个不同的免疫原性区域。在同一表位作图系统中检测了10份人类HIV-1阳性血清，其中7份与四个免疫原性区域有反应。人类血清识别的两个nef特异性表位与鼠单克隆抗体定义的表位重叠。在多种测试系统中研究了单克隆抗体与重组nef蛋白以及感染和未感染细胞的反应性。针对nef和细胞蛋白的同源区域对结果进行了讨论。
