Romero M, Mosquera J, Rodríguez-Iturbe B
Instituto de Investigaciones Clínicas, Universidad del Zulia, Venezuela.
Nephrol Dial Transplant. 1997 Jan;12(1):174-9. doi: 10.1093/ndt/12.1.174.
Reactive oxygen radicals are probably involved in the pathogenesis of human and experimental models of renal disease, yet current methods are inadequate to quantify and identify the cells producing reactive oxygen radicals.
We used the nitroblue tetrazolium reaction to determine superoxide anion production in glomerular cells in phorbol myristate-stimulated glomerular suspensions and in isolated glomeruli from rats with nephrotoxic nephritis, ureteral obstruction, and puromycin aminonucleoside nephrosis. We were also able to identify these nitroblue tetrazolium + cells using specific appropriate antibodies. When the technique was tested in conditions known to increase reactive oxygen radicals, as phorbol myristate-stimulated glomeruli and glomeruli from animals with nephrotoxic nephritis and ureteral obstruction, increased number of nitroblue tetrazolium + cells were found. These cells were identified as glomerular intrinsic cells (Thy-1 +) or infiltrating leukocytes (leukocyte common antigen + or antineutrophil +).
This method may be useful to determine cells participating in glomerular damage induced by reactive oxygen radicals.
活性氧自由基可能参与人类和实验性肾脏疾病模型的发病机制，但目前的方法不足以量化和识别产生活性氧自由基的细胞。
我们使用硝基蓝四唑反应来测定佛波醇肉豆蔻酸酯刺激的肾小球悬液以及来自患有肾毒性肾炎、输尿管梗阻和嘌呤霉素氨基核苷肾病大鼠的分离肾小球中肾小球细胞中超氧阴离子的产生。我们还能够使用特异性合适抗体识别这些硝基蓝四唑阳性细胞。当该技术在已知会增加活性氧自由基的条件下进行测试时，如佛波醇肉豆蔻酸酯刺激的肾小球以及来自患有肾毒性肾炎和输尿管梗阻动物的肾小球，发现硝基蓝四唑阳性细胞数量增加。这些细胞被鉴定为肾小球固有细胞（Thy-1阳性）或浸润性白细胞（白细胞共同抗原阳性或抗中性粒细胞阳性）。
该方法可能有助于确定参与活性氧自由基诱导的肾小球损伤的细胞。
