He J, Li H, Li X
Department of Chemistry, Zhanjiang Normal College, People's Republic of China.
Ann Ist Super Sanita. 1996;32(3):375-80.
In this paper, 1-O-hexadecyl and 1-O-octadecyl-2-acetyl-sn-glycero-3- phosphorylcholine (C16-AGEPC and C18-AGEPC) in the platelet activating factor of human blood were analyzed by micellar electrokinetic capillary chromatography (MECC) with indirect ultraviolet absorption detection at 254 nm. The optimum running buffer for the separation contained 50 mmol/l sodium dodecy sulphate, 20 mmol/l potassium hydrogen phthalate, 10 mmol/l borax and 3 mol/l urea (pH 6.8). The separation was completed within 10 min. The detection limits of C16-AGEPC and C18-AGEPC were the same, i.e. 60 ng/ml (k = 3). The analytical precision (n = 6) was 2.8-3.0% and 1.4-1.7% for the determination (peak height mode) and for the measurement of the migration times, respectively. The application of this method to the clinical samples was demonstrated.
本文采用胶束电动毛细管色谱法（MECC），于254nm处进行间接紫外吸收检测，对人血血小板活化因子中的1-O-十六烷基和1-O-十八烷基-2-乙酰基-sn-甘油-3-磷酸胆碱（C16-AGEPC和C18-AGEPC）进行了分析。分离的最佳运行缓冲液含有50mmol/l十二烷基硫酸钠、20mmol/l邻苯二甲酸氢钾、10mmol/l硼砂和3mol/l尿素（pH 6.8）。分离在10分钟内完成。C16-AGEPC和C18-AGEPC的检测限相同，即60ng/ml（k = 3）。测定（峰高模式）和迁移时间测量的分析精密度（n = 6）分别为2.8 - 3.0%和1.4 - 1.7%。证明了该方法在临床样本中的应用。
