López C, Carballal M J, Azevedo C, Villalba A
Centre of Marine Research, Ministry of Fisheries and Aquaculture of Galicia, Vilagarcía de Arousa, 36600, Spain.
J Invertebr Pathol. 1997 Jan;69(1):51-7. doi: 10.1006/jipa.1996.4639.
Hemocytes play an important role in internal defence in molluscs. The morphology of hemolymph cells was studied for the first time in Ruditapes decussatus. Two main types of hemocytes (hyalinocytes and granulocytes) exist in R. decussatus. Three types of granulocytes were identified by light microscopy, in accordance with the presence of basophilic or acidophilic granules or a mixture of both in the cytoplasm. The existence of hyalinocytes and granulocytes was confirmed by electron microscopy. Some monoclonal antibodies (MABs) raised against hemocytes of Crassostrea gigas showed cross-reactivity with the total population of hemocytes of R. decussatus; however, none of the MABs raised against hemocytes of Mytilus edulis showed cross-reactivity. The MABs assayed did not allow us to distinguish hemocyte subpopulations.
血细胞在软体动物的内部防御中发挥着重要作用。首次对波纹巴非蛤的血淋巴细胞形态进行了研究。波纹巴非蛤存在两种主要类型的血细胞（透明细胞和颗粒细胞）。通过光学显微镜，根据细胞质中嗜碱性或嗜酸性颗粒或两者混合的情况，鉴定出三种类型的颗粒细胞。电子显微镜证实了透明细胞和颗粒细胞的存在。一些针对太平洋牡蛎血细胞产生的单克隆抗体与波纹巴非蛤的血细胞总体呈现交叉反应；然而，针对紫贻贝血细胞产生的单克隆抗体均未显示出交叉反应。所检测的单克隆抗体无法使我们区分血细胞亚群。
