Instituto de Investigaciones Marinas, CSIC, C/Eduardo Cabello, 6, 36208 Vigo, Spain.
Fish Shellfish Immunol. 2012 Jan;32(1):69-78. doi: 10.1016/j.fsi.2011.10.019. Epub 2011 Oct 29.
The morphology and functionality of Ruditapes decussatus haemocytes have been characterized by light microscopy and flow cytometry, leading to the identification of three different cellular subpopulations. Granulocytes were the largest cells, the hyalinocytes were smaller and contained fewer granules and the intermediate cells showed a size similar to hyalinocytes and a higher number of granules. The phagocytosis of different particles and the associated production of oxygen radicals were measured by flow cytometric methods. Granulocytes were the most active cells, followed by the intermediate cells and hyalinocytes. The effect of stimulation of haemocytes with lipopolysaccharide (LPS), with a heat inactivated bacterial mixture or with the infection of Vibrio splendidus on the cell viability and the expression of selected immune-related genes were studied. While significant low levels of damaged cells were registered in LPS-stimulated cells, the treatment with dead bacteria or V. splendidus reduced cell viability 1 h, 3 h and 6 h after treatment. The stimulation of haemocytes with LPS and dead bacteria induced changes in the expression of defender against cell death (DAD-1), thrombin, prosaposin, inhibitor of apoptosis (IAP), factor B and C3 complement component.
利用光学显微镜和流式细胞术对泥蚶血细胞的形态和功能进行了研究，鉴定出了三种不同的细胞亚群。粒细胞是最大的细胞，透明细胞较小，颗粒较少，而中间细胞的大小与透明细胞相似，颗粒数量较多。通过流式细胞术方法测量了不同颗粒的吞噬作用和相关活性氧的产生。粒细胞是最活跃的细胞，其次是中间细胞和透明细胞。研究了用脂多糖（LPS）、热灭活细菌混合物或灿烂弧菌感染刺激血细胞对细胞活力和选定免疫相关基因表达的影响。虽然在 LPS 刺激的细胞中记录到明显的低水平损伤细胞，但在用死细菌或 V. splendidus 处理后 1、3 和 6 小时，细胞活力降低。用 LPS 和死细菌刺激血细胞诱导了细胞死亡防御因子（DAD-1）、凝血酶、前体酶、凋亡抑制剂（IAP）、因子 B 和 C3 补体成分的表达变化。
