Karawajczyk M, Höglund M, Ericsson J, Venge P
Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
Br J Haematol. 1997 Feb;96(2):259-65. doi: 10.1046/j.1365-2141.1997.d01-2045.x.
Any influence of G-CSF on eosinophils is mostly negative, although reports which have studied this relationship are few with varied results. The aim of this study was to investigate the influence of G-CSF administration to healthy subjects on eosinophils in peripheral blood. Blood eosinophil counts, serum levels of eosinophil cationic protein (ECP), eosinophil peroxidase (EPO) and eosinophil protein X (EPX), as well as cell morphology were studied. 14 healthy volunteers received 7.5 microg (n = 8) or 10 microg/kg body weight (n = 6) G-CSF daily for six consecutive days. ECP and EPX were assessed by specific RIAs and EPO by a specific FEIA. Cell morphology was examined by electron microscopy. During G-CSF administration, eosinophil counts increased from 0.22 +/- 0.04 x 10(9)/l to 0.61 +/- 0.098 x 10(9)/l (P = 0.001), serum ECP from 12.39 +/- 2.45 microg/l to 61.82 +/- 7.38 microg/l (P = 0.0014), serum EPX from 28.05 +/- 4.54 microg/l to 87.86 +/- 9.84 microg/l (P = 0.002) and serum EPO from 8.89 +/- 2.2 microg/l to 19.98 +/- 5.1 microg/l (P = 0.003). All variables returned gradually to initial values after discontinuation of G-CSF. Distinct changes in the morphology of secondary granules were observed 24 h after G-CSF administration. The granules became irregular and their matrix less electron dense. We conclude that administration of G-CSF to healthy humans increases the number of circulating eosinophils and affects the mobilization of eosinophil granule proteins.
粒细胞集落刺激因子（G-CSF）对嗜酸性粒细胞的影响大多是负面的，尽管研究这种关系的报告很少且结果各异。本研究的目的是调查对健康受试者施用G-CSF对外周血嗜酸性粒细胞的影响。研究了血液嗜酸性粒细胞计数、嗜酸性粒细胞阳离子蛋白（ECP）、嗜酸性粒细胞过氧化物酶（EPO）和嗜酸性粒细胞蛋白X（EPX）的血清水平以及细胞形态。14名健康志愿者连续6天每天接受7.5微克（n = 8）或10微克/千克体重（n = 6）的G-CSF。通过特定的放射免疫分析（RIA）评估ECP和EPX，通过特定的荧光酶免疫分析（FEIA）评估EPO。通过电子显微镜检查细胞形态。在施用G-CSF期间，嗜酸性粒细胞计数从0.22±0.04×10⁹/升增加到0.61±0.098×10⁹/升（P = 0.001），血清ECP从12.39±2.45微克/升增加到61.82±7.38微克/升（P = 0.0014），血清EPX从28.05±4.54微克/升增加到87.86±9.84微克/升（P = 0.002），血清EPO从8.89±2.2微克/升增加到19.98±5.1微克/升（P = 0.003）。停用G-CSF后，所有变量逐渐恢复到初始值。在施用G-CSF 24小时后观察到次级颗粒形态的明显变化。颗粒变得不规则，其基质的电子密度降低。我们得出结论，对健康人施用G-CSF会增加循环嗜酸性粒细胞的数量，并影响嗜酸性粒细胞颗粒蛋白的动员。
