Owens J, Robbins C A, Wenzel H J, Schwartzkroin P A
Department of Physiology and Biophysics, University of Washington, Seattle 98195-6470, USA.
Ann Neurol. 1997 Feb;41(2):187-99. doi: 10.1002/ana.410410210.
Perinatal hypoxia is associated with both seizures arising acutely and the subsequent development of temporal lobe epilepsy (as determined retrospectively). We therefore attempted to identify acute and chronic morphological and/or electrophysiological hippocampal pathologies associated with experimentally induced hypoxia in immature rats. Pups were exposed to 15 minutes of hypoxia on 3 successive days (starting on postnatal day 8; P8), or to 60 minutes of hypoxia on P10 with either one or multiple hypoxia-induced seizures. For animals experiencing multiple seizures, flurothyl seizure threshold was significantly lower than that of controls at 60 to 80 days, but not at 10 days, after hypoxia. Acutely, there was a treatment-related increase in the number and the density of pyknotic dentate and hilar neurons, in particular in animals experiencing multiple seizures. However, 60 to 80 days after the multiple-seizure protocol, the number of dentate and hilar neurons did not differ between control and experimental animals. Electrophysiological measures of pyramidal cell properties showed no striking difference between experimental and control animals at any time point. These results indicate that early postnatal hypoxia and hypoxia-induced seizure episodes decrease seizure threshold in the adult but produce minimal acute or chronic morphological or functional changes in the hippocampus.
围产期缺氧与急性发作的癫痫以及随后发生的颞叶癫痫（通过回顾性研究确定）均有关联。因此，我们试图确定与幼鼠实验性诱导缺氧相关的急性和慢性海马形态学和/或电生理学病变。幼鼠在连续3天（从出生后第8天即P8开始）暴露于15分钟的缺氧环境，或者在P10时暴露于60分钟的缺氧环境，伴有一次或多次缺氧诱导的癫痫发作。对于经历多次癫痫发作的动物，在缺氧后60至80天，氟烷惊厥阈值显著低于对照组，但在10天时无差异。急性情况下，固缩的齿状回和海马神经元数量及密度出现与治疗相关的增加，特别是在经历多次癫痫发作的动物中。然而，在多次癫痫发作方案实施60至80天后，对照组和实验组动物的齿状回和海马神经元数量并无差异。锥体细胞特性的电生理测量在任何时间点实验组和对照组动物之间均未显示出显著差异。这些结果表明，出生后早期缺氧和缺氧诱导的癫痫发作会降低成年期的惊厥阈值，但在海马中产生的急性或慢性形态学或功能变化极小。
