Rakowicz-Szulczynska E M, Markowski M, Mackiewicz A, Karczewska A, Snyder W, McIntosh D G, Kapcinska M, Smith M L
Department of Obstetrics and Gynecology, University of Nebraska Eppley Cancer Center, Omaha, USA.
Cancer Lett. 1997 Jan 15;112(1):93-101. doi: 10.1016/S0304-3835(96)04550-8.
Breast cancer antigens RAK-p120, -p42, -p25 were detected in 100% of breast cancer cases tested (71 cases). Only 10% of adjacent tissue cases tested positive for all three cancer antigens, and 17.5% of the cases tested positive for two antigens only. Eighty-five percent of histologically normal breast tissue samples, isolated either from breast cancer patients or patients with advanced fibrocystic disease, tested RAK-negative, with the exception of low expression of p25, observed in some patients. Polymerase chain reaction (PCR) with HIV-1 gp 41-derived primers revealed cancer-associated DNA fragments of similar size (140 bp) as in HIV-1 genome. Fifty-four percent of cancer adjacent tissues, and 50% of malignancy-free breast tissue samples, tested PCR-negative. It is suggested that genetic predisposition to cancer may be associated with the presence of RAK genes, while expression of RAK antigens marks an already ongoing process of malignant changes.
在检测的所有乳腺癌病例（71例）中均检测到乳腺癌抗原RAK-p120、-p42、-p25。仅10%的相邻组织病例对所有三种癌抗原检测呈阳性，17.5%的病例仅对两种抗原检测呈阳性。从乳腺癌患者或晚期纤维囊性疾病患者中分离出的85%组织学正常的乳腺组织样本检测RAK呈阴性，不过在一些患者中观察到p25有低表达。用HIV-1 gp 41衍生引物进行的聚合酶链反应（PCR）显示，与HIV-1基因组中大小相似（140 bp）的癌症相关DNA片段。54%的癌旁组织和50%无恶性肿瘤的乳腺组织样本PCR检测呈阴性。提示癌症的遗传易感性可能与RAK基因的存在有关，而RAK抗原的表达标志着恶性变化已经在进行。
