Rakowicz-Szulczynska E M, Jackson B, Szulczynska A M, Smith M
Departments of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Clin Diagn Lab Immunol. 1998 Sep;5(5):645-53. doi: 10.1128/CDLI.5.5.645-653.1998.
RAK antigens p120, p42, and p25 exhibit molecular and immunological similarity to the proteins encoded by human immunodeficiency virus type 1 (HIV-1) and are expressed by 95% of breast and gynecological cancer cases in women and prostate cancer cases in men. The binding of an epitope-specific anti-HIV-1 gp120 monoclonal antibody (MAb) (amino acids 308 to 322) to cancer RAK antigens has been found to be inhibited by a peptide derived from variable loop V3 of HIV-1. Breast cancer DNAs of 40 patients were PCR amplified with HIV-1 gp41-derived primers, and all of the samples were found to be positive. The DNA fragments amplified in seven blindly selected breast cancer samples were sequenced. The breast cancer DNA sequences showed at least 90% homology to the HIV-1 gene for gp41. Antisense oligonucleotides complementary to the HIV-1-like sequences inhibited reverse transcriptase activity and inhibited the growth of breast cancer cells in vitro. Viral particles detected in breast cancer cell lines were strongly immunogold labeled with the anti-HIV-1 gp120 MAb. The results obtained strongly suggest that the long-postulated breast cancer virus may, in fact, be related to HIV-1.
RAK抗原p120、p42和p25在分子和免疫学上与1型人类免疫缺陷病毒（HIV-1）编码的蛋白质相似，在95%的女性乳腺癌和妇科癌症病例以及男性前列腺癌病例中表达。已发现一种表位特异性抗HIV-1 gp120单克隆抗体（MAb）（氨基酸308至322）与癌症RAK抗原的结合受到源自HIV-1可变环V3的肽的抑制。用HIV-1 gp41衍生引物对40例患者的乳腺癌DNA进行PCR扩增，发现所有样本均为阳性。对7个随机选择的乳腺癌样本中扩增的DNA片段进行测序。乳腺癌DNA序列与HIV-1 gp41基因显示出至少90%的同源性。与HIV-1样序列互补的反义寡核苷酸在体外抑制逆转录酶活性并抑制乳腺癌细胞的生长。在乳腺癌细胞系中检测到的病毒颗粒被抗HIV-1 gp120 MAb强烈免疫金标记。所得结果强烈表明，长期以来假定的乳腺癌病毒实际上可能与HIV-1有关。
