Strohl K P, Thomas A J, St Jean P, Schlenker E H, Koletsky R J, Schork N J
Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA.
J Appl Physiol (1985). 1997 Jan;82(1):317-23. doi: 10.1152/jappl.1997.82.1.317.
We examined ventilation and metabolism in four rat strains with variation in traits for body weight and/or blood pressure regulation. Sprague-Dawley [SD; 8 males (M), 8 females (F)], Brown Norway (BN; 10 M, 11 F), and Zucker (Z; 11 M, 12 F) rats were compared with Koletsky (K; 11 M, 11 F) rats. With the use of noninvasive plethysmography, frequency, tidal volume, minute ventilation (VE), O2 consumption, and CO2 production were derived at rest during normoxia (room air) and during the 5th minute of exposure to each of the following: hyperoxia (100% O2), hypoxia (10% O2-balance N2), and hypercapnia (7% CO2-balance O2). Statistical methods probed for strain and sex effects, with covariant analysis by body weight, length, and body mass. During resting breathing, strain effects were found with respect to both frequency (BN, Z > K, SD) and tidal volume (SD > BN, Z) but not to VE. Sex influenced frequency (F > M) alone. Z rats had higher values for O2 consumption, CO2 production, and respiratory quotient than the other three strains, with no independent effect by sex. During hyperoxia, frequency was greater in BN and Z than in SD or K rats; SD rats had a larger tidal volume than BN or Z rats; Z rats had a greater VE than K rats; and M had a larger tidal volume than F. Strain differences persisted during hypercapnia, with Z rats exhibiting the highest frequency and VE values. During hypoxic exposure, strain effects were found to influence VE (SD > K, Z), frequency (BN > K), and tidal volume (SD > BN, K, Z). Body mass was only a modest predictor of VE during normoxia, of both VE and tidal volume with hypoxia, hypercapnia, or hyperoxia, and of frequency during hypercapnia. We conclude that strain of rats, more than their body mass or sex, has major and different influences on metabolism, the pattern and level of ventilation during air breathing, and ventilation during acute exposure to hypercapnia or hypoxia.
我们研究了四种在体重和/或血压调节特征方面存在差异的大鼠品系的通气和代谢情况。将斯普拉格-道利大鼠[SD；8只雄性（M），8只雌性（F）]、挪威棕色大鼠（BN；10只M，11只F）和 Zucker 大鼠（Z；11只M，12只F）与科莱茨基大鼠（K；11只M，11只F）进行比较。使用无创体积描记法，在常氧（室内空气）状态下以及暴露于以下每种情况的第5分钟时，得出静息状态下的频率、潮气量、分钟通气量（VE）、耗氧量和二氧化碳产生量：高氧（100% O₂）、低氧（10% O₂ - 平衡氮气）和高碳酸血症（7% CO₂ - 平衡氧气）。统计方法探究品系和性别效应，并通过体重、体长和体重进行协方差分析。在静息呼吸期间，发现品系对频率（BN、Z > K、SD）和潮气量（SD > BN、Z）有影响，但对 VE 无影响。性别仅影响频率（F > M）。Z 大鼠的耗氧量、二氧化碳产生量和呼吸商的值高于其他三个品系，且不受性别独立影响。在高氧期间，BN 和 Z 大鼠的频率高于 SD 或 K 大鼠；SD 大鼠的潮气量大于 BN 或 Z 大鼠；Z 大鼠的 VE 大于 K 大鼠；雄性的潮气量大于雌性。在高碳酸血症期间，品系差异仍然存在，Z 大鼠表现出最高的频率和 VE 值。在低氧暴露期间，发现品系效应会影响 VE（SD > K、Z）、频率（BN > K）和潮气量（SD > BN、K、Z）。体重在常氧状态下仅是 VE 的适度预测指标，在低氧、高碳酸血症或高氧状态下对 VE 和潮气量以及高碳酸血症期间的频率而言也是如此。我们得出结论，大鼠品系对代谢、空气呼吸期间通气的模式和水平以及急性暴露于高碳酸血症或低氧期间的通气的影响，比其体重或性别更为显著且不同。
