Nürnberger T, Wirtz W, Nennstiel D, Hahlbrock K, Jabs T, Zimmermann S, Scheel D
Abt. Stress- und Entwicklungsbiologie, Institut für Pflanzenbiochemie, Halle.
J Recept Signal Transduct Res. 1997 Jan-May;17(1-3):127-36. doi: 10.3109/10799899709036598.
Disease resistance in plant/pathogen interactions requires sensitive and specific recognition mechanisms for pathogen-derived signals in plants. Cultured parsley (Petroselinum crispum) cells respond to treatment with a crude cell wall preparation derived from the phytopathogenic fungus Phytophthora sojae with transcriptional activation of the same set of defense-related genes as are activated in parsley leaves upon infection with fungal spores. A 13 amino acid core sequence (Pep-13) of a 42 kDa fungal cell wall glycoprotein was identified, which stimulates the same responses as the crude cell wall elicitor, namely macroscopic Ca2+ and H(+)-influxes, effluxes of K(+)- and Cl- ions, production of active oxygen species (oxidative burst), defense-related gene activation, and formation of antifungal phytoalexins. Using [125I]Tyr-Pep-13 as ligand in binding assays, a single-class high-affinity binding site in parsley microsomal membranes and protoplasts could be detected. Binding was specific, saturable, and reversible. By chemical crosslinking, a 91 kDa parsley plasma membrane protein was identified to be the receptor of the peptide elicitor. Isolation of this receptor protein involved in pathogen defense in plants is under way.
植物/病原体相互作用中的抗病性需要植物对病原体衍生信号具备灵敏且特异的识别机制。培养的欧芹（Petroselinum crispum）细胞在用源自植物致病真菌大豆疫霉的粗细胞壁制剂处理后，会激活与用真菌孢子感染欧芹叶片时相同的一组防御相关基因的转录。已鉴定出一种42 kDa真菌细胞壁糖蛋白的13个氨基酸的核心序列（Pep - 13），它能引发与粗细胞壁激发子相同的反应，即宏观的Ca2+和H(+)内流、K(+)和Cl(-)离子外流、活性氧物质的产生（氧化爆发）、防御相关基因的激活以及抗真菌植保素的形成。在结合试验中使用[125I]Tyr - Pep - 13作为配体，可在欧芹微粒体膜和原生质体中检测到单类高亲和力结合位点。结合具有特异性、饱和性和可逆性。通过化学交联，鉴定出一种91 kDa的欧芹质膜蛋白为该肽激发子的受体。参与植物病原体防御的这种受体蛋白的分离工作正在进行中。
