Hahlbrock K, Scheel D, Logemann E, Nürnberger T, Parniske M, Reinold S, Sacks W R, Schmelzer E
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Cologne, Germany.
Proc Natl Acad Sci U S A. 1995 May 9;92(10):4150-7. doi: 10.1073/pnas.92.10.4150.
We have used suspension-cultured parsley cells (Petroselinum crispum) and an oligopeptide elicitor derived from a surface glycoprotein of the phytopathogenic fungus Phytophthora megasperma f.sp. glycinea to study the signaling pathway from elicitor recognition to defense gene activation. Immediately after specific binding of the elicitor by a receptor in the plasma membrane, large and transient increases in several inorganic ion fluxes (Ca2+, H+, K+, Cl-) and H2O2 formation are the first detectable plant cell responses. These are rapidly followed by transient changes in the phosphorylation status of various proteins and by the activation of numerous defense-related genes, concomitant with the inactivation of several other, non-defense-related genes. A great diversity of cis-acting elements and trans-acting factors appears to be involved in elicitor-mediated gene regulation, similar to the apparently complex nature of the signal transduced intracellularly. With few exceptions, all individual defense responses analyzed in fungus-infected parsley leaves have been found to be closely mimicked in elicitor-treated, cultured parsley cells, thus validating the use of the elicitor/cell culture system as a valuable model system for these types of study.
我们利用悬浮培养的欧芹细胞（皱叶欧芹）和一种源自植物致病真菌大豆疫霉表面糖蛋白的寡肽激发子，来研究从激发子识别到防御基因激活的信号传导途径。激发子与质膜中的受体特异性结合后，几种无机离子通量（Ca2+、H+、K+、Cl-）和H2O2的生成会立即出现大幅且短暂的增加，这是植物细胞首先可检测到的反应。随后迅速出现各种蛋白质磷酸化状态的短暂变化以及众多防御相关基因的激活，同时一些其他非防御相关基因失活。顺式作用元件和反式作用因子的种类繁多，似乎都参与了激发子介导的基因调控，这与细胞内转导信号的明显复杂性类似。除了少数例外，在真菌侵染的欧芹叶片中分析的所有个体防御反应，在激发子处理的悬浮培养欧芹细胞中都被发现能被紧密模拟，从而验证了激发子/细胞培养系统作为这类研究的有价值模型系统的用途。
