MacAllan D, Sohal J, Walker C, Hill D, Moore M
Xenova Ltd, Slough, Berkshire, U.K.
J Recept Signal Transduct Res. 1997 Jan-May;17(1-3):521-9. doi: 10.3109/10799899709036625.
The TNF alpha receptor is a major therapeutic target since over production of TNF alpha plays a key role in the development of septic shock following bacterial infection and has been implicated in the pathogenesis of many inflammatory disorders such as rheumatoid arthritis. To screen our NATCHEM Libraries for novel natural product inhibitors of this target we have developed a sensitive immobilised TNF alpha receptor binding assay based on a commercially available recombinant soluble TNF alpha receptor (p55) and [125I]-TNF alpha.
肿瘤坏死因子α受体是一个主要的治疗靶点，因为肿瘤坏死因子α的过量产生在细菌感染后脓毒症休克的发展中起关键作用，并且与许多炎症性疾病如类风湿性关节炎的发病机制有关。为了从我们的NATCHEM文库中筛选该靶点的新型天然产物抑制剂，我们基于市售的重组可溶性肿瘤坏死因子α受体（p55）和[125I]-肿瘤坏死因子α开发了一种灵敏的固定化肿瘤坏死因子α受体结合测定法。
