采用高效液相色谱法测定人血浆和尿液中新型抗溃疡药物YJA - 20379 - 2的含量。
Determination of a new antiulcer agent, YJA-20379-2, in human plasma and urine by high-performance liquid chromatography.
作者信息
Yoon W H, Kim S H, Chung S Y, Chang M S, Choi W S, Sohn S K, Lee M G
机构信息
College of Pharmacy, Seoul National University, Shinlim-Dong, Kwanak-Gu, Korea.
出版信息
Res Commun Mol Pathol Pharmacol. 1996 Dec;94(3):317-22.
A high-performance liquid chromatographic method was developed for the determination of a new antiulcer agent, YJA-20379-2, in human plasma and urine. The sample preparation was simple: 2.5-volume of acetonitrile was added to the biological sample to deproteinize. A 50-microliter aliquot of the supernatant was injected onto a C18 reversed-phase column. The mobile phase employed was methanol-0.1M Sørensen phosphate buffer of pH 7.0-H2O (75:2:25, v/v/v), and was run at a flow-rate of 1.0 ml/min. The column effluent was monitored by ultraviolet detector at 295 nm. The retention time for YJA-20379-2 was approximately 7.0 min. The detection limits for YJA-20379-2 in human plasma and urine were both 100 ng/ml. The coefficients of variation of the assay (within-day and between-day) were generally low (below 9.16%) for both the human plasma and urine. No interference from endogenous substances was found.
建立了一种高效液相色谱法，用于测定人血浆和尿液中的新型抗溃疡药物YJA - 20379 - 2。样品制备简单：向生物样品中加入2.5倍体积的乙腈进行去蛋白处理。取50微升上清液注入C18反相柱。所用流动相为甲醇 - 0.1M pH 7.0的索伦森磷酸盐缓冲液 - 水（75:2:25，v/v/v），流速为1.0毫升/分钟。柱流出物通过紫外检测器在295纳米处进行监测。YJA - 20379 - 2的保留时间约为7.0分钟。YJA - 20379 - 2在人血浆和尿液中的检测限均为100纳克/毫升。该测定法（日内和日间）的变异系数对于人血浆和尿液而言通常都很低（低于9.16%）。未发现内源性物质的干扰。
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