Kim S H, Kim S H, Lee S D, Kim W B, Lee M G, Kim N D
College of Pharmacy, Seoul National University, South Korea.
Res Commun Mol Pathol Pharmacol. 1997 Aug;97(2):165-70.
A high-performance liquid chromatographic method was developed for the determination of a new antiulcer agent, eupatilin, in rat plasma, urine, bile, and liver homogenate. The method involved deproteinization of biological sample with the same volume of acetonitrile. A 100 microl aliquot of the supernatant was injected onto a C18 reversed-phase column. The mobile phase employed was ammonium acetate buffer (1% ammonium acetate and 0.5% acetic acid) - acetonitrile (58:42, v/v) and the flow rate was 1.0 ml/min. The column effluent was monitored by a ultraviolet detector set at 350 nm. The retention time for eupatilin was approximately 6.5 min. The detection limits for eupatilin in rat plasma, urine, bile, and liver homogenate were 50, 50, 100, and 100 ng/ml, respectively. The coefficients of variation of the assay were generally low (below 7.46%) for rat plasma, urine, bile, and liver homogenate. No interferences from endogenous substances were observed.
建立了一种高效液相色谱法，用于测定大鼠血浆、尿液、胆汁和肝匀浆中一种新型抗溃疡药物灯盏乙素的含量。该方法采用相同体积的乙腈对生物样品进行去蛋白处理。取100微升上清液注入C18反相柱。所用流动相为醋酸铵缓冲液（1%醋酸铵和0.5%醋酸）-乙腈（58:42，v/v），流速为1.0毫升/分钟。柱流出物用设定在350纳米的紫外检测器进行监测。灯盏乙素的保留时间约为6.5分钟。灯盏乙素在大鼠血浆、尿液、胆汁和肝匀浆中的检测限分别为50、50、100和100纳克/毫升。该测定方法在大鼠血浆、尿液、胆汁和肝匀浆中的变异系数通常较低（低于7.46%）。未观察到内源性物质的干扰。
