Schneider-Broussard R, Neigel J E
Department of Biology, University of Southwestern Louisiana, Lafayette, USA.
Mol Biol Evol. 1997 Feb;14(2):156-65. doi: 10.1093/oxfordjournals.molbev.a025748.
Two DNA sequences that appear to be homologous to large-subunit mitochondrial ribosomal RNA genes have been identified in the stone crabs Menippe mercenaria and M. adina. Amplification from whole genomic DNA by polymerase chain reaction (PCR) with oligonucleotide primers based on conserved portions of large-subunit mitochondrial rRNA genes consistently amplified two products of similar length (565 and 567 bp). These products differed at 3% of their nucleotide bases, and could be distinguished by a HindIII site. Only one of these sequences (designated the A sequence) was detected by PCR in purified mitochondrial DNA. The other (designated the B sequence) hybridized to total genomic DNA at a level consistent with a nuclear genome location. It is unlikely that the type B product would have been recognized as a nuclear copy by examination of its sequence alone. This is the first report of a mitochondrial gene sequence translocated into the nuclear genome of a crustacean.
在石蟹梅氏新短桨蟹（Menippe mercenaria）和阿氏新短桨蟹（M. adina）中，已鉴定出两个与大 subunit 线粒体核糖体 RNA 基因似乎同源的 DNA 序列。基于大 subunit 线粒体 rRNA 基因保守部分的寡核苷酸引物，通过聚合酶链反应（PCR）从全基因组 DNA 中扩增，始终扩增出两个长度相似的产物（565 和 567 碱基对）。这些产物在 3%的核苷酸碱基上存在差异，并且可以通过一个 HindIII 位点加以区分。在纯化的线粒体 DNA 中，通过 PCR 仅检测到这些序列中的一个（命名为 A 序列）。另一个（命名为 B 序列）与全基因组 DNA 杂交的水平与核基因组定位一致。仅通过检查其序列，不太可能将 B 型产物识别为核拷贝。这是关于线粒体基因序列易位到甲壳类动物核基因组中的首次报道。
