Wang A, Yoshimi N, Ino N, Tanaka T, Mori H
Department of Pathophysiology, Zhejiang Medical University, Hangzhou, China.
J Cancer Res Clin Oncol. 1997;123(2):124-7. doi: 10.1007/BF01269891.
The expression of the human cyclin B1 gene was investigated with Western blot analysis in human colorectal carcinomas and in adjacent non-neoplastic colorectal mucosas. Out of 41 cancers, 36 (88% of patients) showed much higher expression of cyclin B1 than did the non-neoplastic mucosa. Proliferating-cell nuclear antigen (PCNA) immunohistochemistry revealed that the labeling indexes of these cancer tissues were 47.3 +/- 11.3% while those of the mucosa were 15.6 +/- 5.5%. Only 5 cancers (12% patients) demonstrated the same expression level of cyclin B1 as the mucosa; however, the PCNA labeling indexes were 42.3 +/- 11% for the cancer tissue, compared to 12.6 +/- 2.4% for the mucosas. Southern blot analysis showed that there was no change of the cyclin B1 gene at the somatic DNA level in spite of its high expression at the protein level. These results proved that majority of colorectal cancers express high levels of cyclin B1, consistent with a high rate of cell proliferation, whereas a small fraction of these cancers lose control of cyclin B1 expression, diverging from their fast cell proliferation.
采用蛋白质印迹分析法对人结直肠癌及其癌旁非肿瘤性结直肠黏膜组织中人类细胞周期蛋白B1基因的表达情况进行了研究。在41例癌症患者中，有36例（占患者总数的88%）的细胞周期蛋白B1表达水平远高于非肿瘤性黏膜组织。增殖细胞核抗原（PCNA）免疫组织化学分析显示，这些癌组织的标记指数为47.3±11.3%，而黏膜组织的标记指数为15.6±5.5%。仅有5例癌症（占患者总数的12%）的细胞周期蛋白B1表达水平与黏膜组织相同；然而，这些癌组织的PCNA标记指数为42.3±11%，而黏膜组织的PCNA标记指数为12.6±2.4%。Southern印迹分析表明，尽管细胞周期蛋白B1在蛋白质水平上呈高表达，但在体细胞DNA水平上其基因并无改变。这些结果证明，大多数结直肠癌中细胞周期蛋白B1呈高水平表达，这与高细胞增殖率相一致，而一小部分此类癌症则失去了对细胞周期蛋白B1表达的控制，与其快速的细胞增殖情况有所不同。
