Giusti G V, Chiarotti M, Passatore M, Gentile V, Fiori A
Forensic Sci. 1977 Sep-Oct;10(2):133-40. doi: 10.1016/0300-9432(77)90106-6.
Swiss male albino mice were treated subcutaneously with the main cannabinoids (CBN, CBD, delta9-THC) at the dosage of 1 mg/kg per day for 30 days, and with the crude resin. At the end of the treatment, after supramaximal stimulation of the sciatic nerve, a significant decrease of both maximal twitch and tetanus tensions was observed in delta9-THC-treated animals; CBD and resin treatment produced some decrease in active tension, while CBN treatment induced an enhancement of the contractile strength. Histology showed lesions interpretable as due to muscular dystrophy. Analysis of protein and hydroxyproline muscular content showed a marked reduction in protein in all treated animals, with a corresponding high increase in hydroxyproline content.
将瑞士雄性白化小鼠皮下注射主要大麻素（大麻酚、大麻二酚、δ9-四氢大麻酚），剂量为每天1毫克/千克，持续30天，并注射粗提树脂。治疗结束时，在对坐骨神经进行超强刺激后，观察到δ9-四氢大麻酚处理的动物的最大抽搐和强直张力均显著降低；大麻二酚和树脂处理使主动张力有所降低，而大麻酚处理则导致收缩强度增强。组织学显示病变可解释为肌肉营养不良所致。蛋白质和羟脯氨酸肌肉含量分析表明，所有处理动物的蛋白质均显著减少，同时羟脯氨酸含量相应大幅增加。
