Champion H C, Wang R, Shenassa B B, Murphy W A, Coy D H, Hellstrom W J, Kadowitz P J
Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA 70112, USA.
Eur J Pharmacol. 1997 Jan 14;319(1):71-5. doi: 10.1016/s0014-2999(96)00924-7.
The present study was undertaken to investigate the effects of intracavernosal injections of adrenomedullin, a novel hypotensive peptide, on penile erection in anesthetized cats. Responses to adrenomedullin were compared to those elicited by intracavernosal injection of the control triple-drug combination (1.65 mg papaverine, 25 micrograms phentolamine, and 0.5 microgram prostaglandin E1). Intracavernosal injections of adrenomedullin in doses of 0.1-1.0 nmol elicited dose-related increases in cavernosal pressure and penile length. The maximal effect of adrenomedullin injection on cavernosal pressure was an 8-fold increase in pressure, which was 74% of that induced by the triple-drug combination. The maximal effect on penile length was a 43% increase when compared to baseline value, which was comparable to that induced by the triple-drug combination. The duration of the peak pressure and total duration of the peptide effect were significantly shorter in response to the 1 nmol dose of adrenomedullin than was observed with the control triple-drug combination. Intracavernous injection of the control triple-drug combination resulted in a significantly greater decrease in systemic arterial blood pressure than did adrenomedullin. Erectile responses to adrenomedullin were not altered following administration of the nitric oxide synthase inhibitor. N omega-nitro-L-arginine, at a time when erectile responses to acetylcholine were significantly reduced. These data demonstrate that intracavernous injection of adrenomedullin induces a short-lived erection in cats that is not due to the release of nitric oxide.
本研究旨在探讨新型降压肽肾上腺髓质素海绵体内注射对麻醉猫阴茎勃起的影响。将肾上腺髓质素的反应与海绵体内注射对照三联药物组合（1.65毫克罂粟碱、25微克酚妥拉明和0.5微克前列腺素E1）所引发的反应进行比较。0.1 - 1.0纳摩尔剂量的肾上腺髓质素海绵体内注射可引起海绵体压力和阴茎长度与剂量相关的增加。肾上腺髓质素注射对海绵体压力的最大效应是压力增加8倍，这是三联药物组合诱导效应的74%。与基线值相比，对阴茎长度的最大效应是增加43%，这与三联药物组合诱导的效应相当。与对照三联药物组合相比，1纳摩尔剂量的肾上腺髓质素引起的峰值压力持续时间和肽效应总持续时间显著缩短。海绵体内注射对照三联药物组合导致的体循环动脉血压下降幅度明显大于肾上腺髓质素。在对乙酰胆碱的勃起反应显著降低时，给予一氧化氮合酶抑制剂Nω-硝基-L-精氨酸后，对肾上腺髓质素的勃起反应未改变。这些数据表明，海绵体内注射肾上腺髓质素可在猫中诱导短暂的勃起，且这一勃起并非由一氧化氮释放所致。
