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NK1.1 + Ly-6C+ 细胞的功能特性

Functional characterization of NK1.1 + Ly-6C+ cells.

作者信息

Sato N, Yahata T, Santa K, Ohta A, Ohmi Y, Habu S, Nishimura T

机构信息

Department of Immunology, Tokai University, School of Medicine, Isehara, Japan.

出版信息

Immunol Lett. 1996 Dec 1;54(1):5-9. doi: 10.1016/s0165-2478(96)02632-6.

DOI:10.1016/s0165-2478(96)02632-6
PMID:9030975
Abstract

It was found that NK1.1+ cells were subdivided by their different expression pattern of Ly-6C antigen. To characterize their functional significance in immunoregulation, we separated NK1.1 + Ly6C+ cells and NK1.1 + Ly-6C- cells from C57BL/6 mouse nylon-passed spleen cells by FACStar. Both NK1.1 + Ly-6C+ and NK1.1 + Ly-6C- cells responded to the stimulation with IL-2 plus IL-12 and showed strong cytotoxicity against YAC-1 cells. However, these cells revealed different ability in terms of IFN-gamma production. Only NK1.1 + Ly-6C+ cells, but not NK1.1 + Ly-6C- cells, cultured with IL-12 alone or IL-2 plus IL-12, produced high levels of IFN-gamma. Flow cytometric analysis demonstrated that NK1.1 + Ly-6C+ cells consisted of NK1.1 + CD3-Ly-6C+ NK cells and NK1.1 + CD3 + Ly-6C+ NKT cells. Therefore, we further separated these two populations from NK1.1 + Ly-6C+ cells to define their functions. Although, both NK1.1 + CD3-Ly-6C+ NK cells and NK1.1 + CD3+ NKT cells showed the same level of cytotoxicity. It was clearly demonstrated that NK1.1 + CD3+Ly-6C+ NKT cells were major immunoregulatory cells to produce IFN-gamma in respond to IL-12 alone or IL-2 plus IL-12.

摘要

研究发现，NK1.1+细胞可根据其Ly-6C抗原的不同表达模式进行细分。为了表征它们在免疫调节中的功能意义，我们通过FACStar从C57BL/6小鼠尼龙滤网过滤的脾细胞中分离出NK1.1 + Ly6C+细胞和NK1.1 + Ly-6C-细胞。NK1.1 + Ly-6C+细胞和NK1.1 + Ly-6C-细胞均对IL-2加IL-12的刺激有反应，并对YAC-1细胞表现出强烈的细胞毒性。然而，这些细胞在干扰素-γ产生方面表现出不同的能力。仅NK1.1 + Ly-6C+细胞，而非NK1.1 + Ly-6C-细胞，单独用IL-12或IL-2加IL-12培养时，会产生高水平的干扰素-γ。流式细胞术分析表明，NK1.1 + Ly-6C+细胞由NK1.1 + CD3-Ly-6C+ NK细胞和NK1.1 + CD3 + Ly-6C+ NKT细胞组成。因此，我们进一步从NK1.1 + Ly-6C+细胞中分离出这两个群体以确定它们的功能。尽管NK1.1 + CD3-Ly-6C+ NK细胞和NK1.1 + CD3+ NKT细胞表现出相同水平的细胞毒性。但清楚地表明，NK1.1 + CD3+Ly-6C+ NKT细胞是主要的免疫调节细胞，在单独对IL-12或IL-2加IL-12的反应中产生干扰素-γ。

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