Kouri J B, Argüello C, Quintero M, Chico A, Ramos M E
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento Patología Experimental, México DF.
Biocell. 1996 Dec;20(3):191-200.
Human samples of articular cartilage from the knee of a clinically classified osteoarthritic patient, assessed by arthroscopy as part of the surgical treatment was studied by light and transmission electron microscopy. This particular case differed from others already reported in the variability of cell phenotype within the aggregates or "clones" frequently present in the osteoarthritic cartilage. The most common morphology of "clonal" cells forming the aggregates were large and rounded with an euchromatic nucleus. The cytoplasm was characterized by the presence of alternately clear and dense sites. At the ultrastructural level it was seen that the clear sites were formed by disrupted intermediates filaments and small particles, and that the dense sites were constituted by the segregation of different organelles of the chondrocytes. In addition, there were atypical aggregates composed only by secretory cells or by degenerating chondrocytes. Furthermore, a complex structure consisting of a very large cell inside a giant lacunae delimited by electron-dense material with small vesicles is described as a novel finding. The variability in the chondrocyte phenotype of the aggregates described here could be an indication of a better prognosis; nevertheless, the follow-up of the evolution of this patient is needed in order to know the final outcome.
对一名经临床分类为骨关节炎患者膝关节的关节软骨人体样本进行了研究，该样本是作为手术治疗的一部分通过关节镜检查获取的，采用光镜和透射电子显微镜进行观察。该特殊病例与已报道的其他病例不同之处在于，骨关节炎软骨中常见的聚集体或“克隆”内细胞表型存在变异性。形成聚集体的“克隆”细胞最常见的形态是大而圆，细胞核呈常染色质。细胞质的特征是存在交替出现的清亮区和致密区。在超微结构水平上可以看到，清亮区由断裂的中间丝和小颗粒形成，致密区由软骨细胞不同细胞器的分离构成。此外，还存在仅由分泌细胞或退变软骨细胞组成的非典型聚集体。此外，还描述了一种复杂结构，即在由电子致密物质界定的巨大腔隙内有一个非常大的细胞，并伴有小泡，这是一项新发现。这里描述的聚集体软骨细胞表型的变异性可能预示着较好的预后；然而，需要对该患者的病情发展进行随访，以了解最终结果。
