Kouri J B, Argüello C, Luna J, Mena R
Department of Experimental Pathology, Centro de Investigación y de Estudios Avanzados, México, D.F.
Microsc Res Tech. 1998 Jan 1;40(1):22-36. doi: 10.1002/(SICI)1097-0029(19980101)40:1<22::AID-JEMT4>3.0.CO;2-Z.
Several microscopical techniques, such as high resolution light microscopy, Normaski microscopy, laser confocal and transmission electron microscopy, were used in a correlative morphological study of human osteoarthritic (OA) cartilage. Emphasis was made on the characterization of chondrocytes heterogeneity observed in this tissue. Novel findings were assessed in the morphological and immunocytological study of the chondrocytes organized in aggregates or "clones" typical of this degenerative disease, consisting of the modification of certain elements of the cytoskeleton that influence changes in the cell shape. Also, the presence of cilia and centrioles found in certain cell raised the question if chondrocytes are able to move and regroup as an alternative mechanism to mitosis in the formation of cell clusters or "clones." The presence of two types of secretory chondrocytes was observed and discussed. The use of a correlative approach of several microscopical techniques in a systematic morphological and immunocytological characterization of chondrocyte population within the fibrillated and nonfibrillated human osteoarthritic cartilage gave complementary information that could be important for a better understanding of the histopathogenesis of OA.
在一项关于人类骨关节炎（OA）软骨的相关形态学研究中，使用了几种显微镜技术，如高分辨率光学显微镜、相差显微镜、激光共聚焦显微镜和透射电子显微镜。重点是对该组织中观察到的软骨细胞异质性进行表征。在对以聚集或“克隆”形式存在的软骨细胞进行形态学和免疫细胞学研究时，评估了新发现，这些聚集或“克隆”是这种退行性疾病的典型特征，包括细胞骨架某些成分的改变，这些改变影响细胞形状的变化。此外，在某些细胞中发现的纤毛和中心粒的存在，引发了软骨细胞是否能够移动和重新聚集，作为细胞簇或“克隆”形成过程中有丝分裂替代机制的问题。观察并讨论了两种类型分泌性软骨细胞的存在。在对纤维化和非纤维化人类骨关节炎软骨内的软骨细胞群体进行系统的形态学和免疫细胞学表征时，使用几种显微镜技术的相关方法提供了补充信息，这对于更好地理解OA的组织发病机制可能很重要。
