Nunn M E, Herberman R B, Holden H T
Int J Cancer. 1977 Sep 15;20(3):381-7. doi: 10.1002/ijc.2910200309.
Lymphocytes from normal mice were found to have cell-mediated cytotoxicity, in a short-term 51Cr release assay, against a variety of non-lymphoid tumor cells as well as against lymphomas. Some of the non-lymphoid tumors were as susceptible to natural cytotoxicity as the standardly used lymphoid lines. Some tissue culture cell lines and in vivo passaged tumor lines were susceptible to lysis, as were some primary virus-induced lymphomas. Tumor which arose in nude mice, which have high levels of natural cytotoxic activity, were all resistant to lysis. In addition to the susceptibility of transformed cells to natural cell-mediated cytotoxicity, some untransformed cultured cells and cells from normal tissues were targets for this mechanism. Low levels of cytotoxicity were seen with normal thymus cells, bone-marrow cells, and short term cultures of macrophages, whereas normal spleen and lymph-node cells were completely resistant to lysis. These results indicate a broader spectrum for mouse natural cell-mediated cytotoxicity reactivity than has been previously recognized.
在一项短期的51Cr释放试验中，发现正常小鼠的淋巴细胞对多种非淋巴细胞肿瘤细胞以及淋巴瘤具有细胞介导的细胞毒性。一些非淋巴细胞肿瘤对自然细胞毒性的敏感性与标准使用的淋巴细胞系相同。一些组织培养细胞系和体内传代的肿瘤系易于被裂解，一些原发性病毒诱导的淋巴瘤也是如此。在具有高水平自然细胞毒性活性的裸鼠中产生的肿瘤均对裂解具有抗性。除了转化细胞对自然细胞介导的细胞毒性敏感外，一些未转化的培养细胞和来自正常组织的细胞也是这种机制的靶标。正常胸腺细胞、骨髓细胞和巨噬细胞短期培养物的细胞毒性水平较低，而正常脾脏和淋巴结细胞对裂解完全具有抗性。这些结果表明，小鼠自然细胞介导的细胞毒性反应的谱比以前认识的更广。
