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本文引用的文献

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A cytoplasmic actin gene from the silkworm Bombyx mori is expressed in tissues of endodermal origin and previtellogenic germ cells of transgenic Drosophila.来自家蚕的一个细胞质肌动蛋白基因在转基因果蝇的内胚层起源组织和卵黄发生前的生殖细胞中表达。
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2
A strong inhibitory element down-regulates SRE-stimulated transcription of the A3 cytoplasmic actin gene of Bombyx mori.一种强抑制元件可下调家蚕A3细胞质肌动蛋白基因的SRE刺激转录。
J Mol Biol. 1997 Jan 24;265(3):266-74. doi: 10.1006/jmbi.1996.0734.
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Transcription revisited: a commentary on the 1995 Cold Spring Harbor Laboratory meeting, "Mechanisms of Eukaryotic Transcription".转录再探讨：对1995年冷泉港实验室会议“真核转录机制”的评论
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Regulation of the P25 gene transcription in the silk gland of Bombyx.家蚕丝腺中P25基因转录的调控
Biol Cell. 1995;84(1-2):43-52. doi: 10.1016/0248-4900(96)81317-7.
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Different behavior of chromatin domains encompassing fibroin heavy-chain gene in active, temporarily inactive, and permanently inactive transcriptional states in silk gland nuclei.
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Immunological identification of the major disulfide-linked light component of silk fibroin.丝素蛋白主要二硫键连接轻链成分的免疫学鉴定
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Transcriptional activation: a complex puzzle with few easy pieces.转录激活：一个几乎没有简单拼图块的复杂谜题。
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ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor.由GAGA转录因子结合介导的热休克启动子处的ATP依赖性核小体破坏。
Nature. 1994 Feb 10;367(6463):525-32. doi: 10.1038/367525a0.
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Analysis of a fushi tarazu autoregulatory element: multiple sequence elements contribute to enhancer activity.腹节基因自动调节元件的分析：多个序列元件对增强子活性有贡献。
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CCAAT box binding protein NF-Y facilitates in vivo recruitment of upstream DNA binding transcription factors.CCAAT盒结合蛋白NF-Y促进体内上游DNA结合转录因子的募集。
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家蚕丝腺特异性因子SGFB与其靶DNA序列的差异结合驱动后细胞限制性表达。

Differential binding of the Bombyx silk gland-specific factor SGFB to its target DNA sequence drives posterior-cell-restricted expression.

作者信息

Horard B, Julien E, Nony P, Garel A, Couble P

机构信息

Centre de Génétique Moléculaire et Cellulaire, CNRS, Université Claude Bernard, Villeurbanne, France.

出版信息

Mol Cell Biol. 1997 Mar;17(3):1572-9. doi: 10.1128/MCB.17.3.1572.

DOI:10.1128/MCB.17.3.1572
PMID:9032285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC231883/
Abstract

The gene encoding the silk protein P25 in Bombyx mori is expressed in the posterior silk gland (PSG) cells and repressed in the middle silk gland (MSG) cells. To identify the factors involved in this transcription-dependent spatial restriction, we examined the P25 chromatin in PSG and MSG nuclei by DNase I-aided ligation-mediated PCR and analyzed the expression of various P25-lacZ constructs in biolistically treated silk glands. P25 promoter activation depends on two cis-acting elements. One coincides with the target sequence of SGFB, a silk gland-specific factor present in all silk gland nuclei, but bound to its target DNA sequence in only PSG cells. The interaction of the other element with a factor that we named PSGF is also exclusive to PSG cells. Placed ahead of a non-P25-related basal promoter, the SGFB and PSGF elements are sufficient to drive posterior-cell transcription. Collectively, our data support the hypothesis that the spatial restriction of P25 expression is driven by the stabilization of SGFB onto its target sequence by the action of PSGF.

摘要

家蚕中编码丝蛋白P25的基因在后部丝腺（PSG）细胞中表达，而在中部丝腺（MSG）细胞中受到抑制。为了确定参与这种转录依赖性空间限制的因素，我们通过DNase I辅助连接介导的PCR检测了PSG和MSG细胞核中的P25染色质，并分析了经生物弹道处理的丝腺中各种P25-lacZ构建体的表达情况。P25启动子的激活依赖于两个顺式作用元件。其中一个与SGFB的靶序列一致，SGFB是一种存在于所有丝腺细胞核中的丝腺特异性因子，但仅在PSG细胞中与其靶DNA序列结合。另一个元件与我们命名为PSGF的因子的相互作用也仅发生在PSG细胞中。置于非P25相关的基础启动子之前，SGFB和PSGF元件足以驱动后部细胞的转录。总的来说，我们的数据支持这样一种假说，即P25表达的空间限制是由PSGF的作用使SGFB稳定在其靶序列上所驱动的。