Schier A F, Gehring W J
Biozentrum, Universität, Basel, Switzerland.
EMBO J. 1993 Mar;12(3):1111-9. doi: 10.1002/j.1460-2075.1993.tb05752.x.
Regulatory sequences or factors involved in the regulation of target genes of Drosophila homeodomain proteins are largely unknown. Here, we identify sequence elements that are involved in the function of the fushi tarazu (ftz) autoregulatory element AE, a direct in vivo target of the homeodomain protein ftz. A systematic deletion analysis of AE in transgenic embryos defines multiple elements that are redundantly involved in enhancer activity. Sequences juxtaposed to ftz binding sites are not strictly required for enhancer function. Several sequence motifs are conserved in other developmentally regulated genes of Drosophila melanogaster and in the AE homologue of Drosophila virilis. The D. virilis AE is functional in D. melanogaster. The sequence motifs identified here are candidate elements contributing to the target specificity of the homeodomain protein ftz.
果蝇同源域蛋白靶基因调控中涉及的调控序列或因子大多未知。在此，我们鉴定了与腹节基因（ftz）自调控元件AE功能相关的序列元件，AE是同源域蛋白ftz在体内的直接靶标。对转基因胚胎中的AE进行系统的缺失分析，确定了多个冗余参与增强子活性的元件。与ftz结合位点并列的序列对于增强子功能并非严格必需。几个序列基序在黑腹果蝇的其他发育调控基因以及果蝇的AE同源物中保守。果蝇的AE在黑腹果蝇中具有功能。这里鉴定的序列基序是有助于同源域蛋白ftz靶标特异性的候选元件。
