Brand J M, Neustock P, Kruse A, Alvarez-Ossorio L, Schnabel A, Kirchner H
Institute of Immunology and Transfusion Medicine, University of Lübeck Medical School, Germany.
Rheumatol Int. 1997;16(5):207-11. doi: 10.1007/BF01330297.
In this study we compared cytokine production and cell proliferation of immunocompetent cells derived from patients with ankylosing spondylitis (AS) to those from healthy blood donors using a whole blood assay. To this end, blood cell cultures were stimulated with the superantigens MAS (Mycoplasma arthritidis supernatant) and staphylococcal enterotoxin B (SEB) and the plant lectins phytohaemagglutinin (PHA) and concanavalin A (Con A). The number of white blood cells (WBC) and lymphocyte subsets were also determined. Cell proliferation and levels of interferon-gamma (IFN-gamma), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) were measured after stimulation with the different mitogens. An ELISA test was used to analyse supernatant cytokine levels. Individuals with AS showed significantly lower IFN-gamma concentrations and markedly lower cell proliferation rates with all tested mitogens than healthy controls, while there was no significant difference in IL-6 synthesis. IL-1 beta levels were slightly impaired in the patient group, but only blood cell cultures stimulates with MAS showed a statistical significance. Furthermore, there was a significant elevation of leucocytes and lymphocytes in patients with AS resulting in higher numbers of CD4-positive cells, which implies a higher CD4:CD8 cell ratio. CD19- and CD8-positive cells were not significantly distinct compared to healthy controls. This deviation in cytokine levels and cell proliferation points to a suppression of T lymphocytes. A disturbed T-lymphocyte function may play a part in the pathogenesis of AS.
在本研究中，我们使用全血检测法，比较了强直性脊柱炎（AS）患者免疫活性细胞与健康献血者免疫活性细胞的细胞因子产生情况和细胞增殖情况。为此，血细胞培养物用超抗原MAS（关节炎支原体上清液）、葡萄球菌肠毒素B（SEB）以及植物凝集素植物血凝素（PHA）和刀豆球蛋白A（Con A）进行刺激。同时还测定了白细胞（WBC）数量和淋巴细胞亚群。在用不同有丝分裂原刺激后，测量细胞增殖以及干扰素-γ（IFN-γ）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的水平。采用酶联免疫吸附测定（ELISA）试验分析上清液细胞因子水平。与健康对照相比，AS患者在所有测试有丝分裂原刺激下，IFN-γ浓度显著降低，细胞增殖率明显较低，而IL-6合成无显著差异。患者组IL-1β水平略有受损，但仅用MAS刺激的血细胞培养物显示有统计学意义。此外，AS患者白细胞和淋巴细胞显著升高，导致CD4阳性细胞数量增加，这意味着CD4:CD8细胞比值更高。与健康对照相比，CD19和CD8阳性细胞无显著差异。细胞因子水平和细胞增殖的这种偏差表明T淋巴细胞受到抑制。T淋巴细胞功能紊乱可能在AS的发病机制中起作用。
