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Studies of DEAE-dextran-mediated gene transfer.

作者信息

Yang Y W, Yang J C

机构信息

School of Pharmacy, College of Medicine, National Taiwan University, Taipei.

出版信息

Biotechnol Appl Biochem. 1997 Feb;25(1):47-51. doi: 10.1111/j.1470-8744.1997.tb00413.x.

DOI:10.1111/j.1470-8744.1997.tb00413.x
PMID:9032935
Abstract

DEAE-dextran-mediated gene transfer was studied for the introduction of pSV2neo DNA into Fisher-rat 3T3 (FR3T3) cells. Zeta (zeta) potentials of the DEAE-dextran-DNA complexes and FR3T3 cells were found to be dependent on the concentration of DEAE-dextran in the medium. The maximum transfection efficiency occurred at a DEAE-dextran/DNA ratio of 50:1 or thereabouts. The interaction between DNA and cells is determined by the adsorption process. The results obtained, along with the correlation between the kinetic adsorption behaviour of 3H-labelled DNA and the transfection efficiency, indicated that adsorption of DEAE-dextran-DNA complexes to the negatively charged cell surfaces, due to electrostatic and dispersion attraction, plays the decisive role in determining the DNA transfection efficiencies.

摘要

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