Kumar Priti, Nagarajan Arvindhan, Uchil Pradeep D
Cold Spring Harb Protoc. 2018 Jul 2;2018(7):2018/7/pdb.top096263. doi: 10.1101/pdb.top096263.
Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured eukaryotic cells. Here, we briefly review the use of DEAE-dextran in transfection.
生物化学转染方法,包括磷酸钙介导的转染和二乙氨基乙基(DEAE)-葡聚糖介导的转染,多年来一直被用于将核酸导入培养的真核细胞。在此,我们简要回顾一下DEAE-葡聚糖在转染中的应用。