Huang S, Afonina I, Miller B A, Beckmann A M
Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Int J Cancer. 1997 Feb 7;70(4):408-11. doi: 10.1002/(sici)1097-0215(19970207)70:4<408::aid-ijc6>3.0.co;2-#.
A substantial body of evidence has confirmed human papillomavirus (HPV) infection as an etiologic agent in human cervical cancer. To evaluate the association between HPV and cervical cancer in Chinese women, we examined tumor specimens from women who lived in Shanghai, People's Republic of China. Biopsies from 40 women, diagnosed with either squamous-cell carcinoma (n = 35) or adenocarcinoma (n = 5) were tested for HPV DNA by PCR. The HPV types present in tumors were determined either by hybridization of PCR products with HPV type-specific probes or by PCR-based sequencing. A total of 35 of the 40 cervical cancer specimens (87.5%) contained HPV DNA. The following distribution and types were detected: 7.5% HPV 16, 10% HPV 18, 20% HPVs 16 and 18, 15% HPV 52, 15% HPV 58, 12.5% HPVs 52 and 58 and 7.5% unclassified HPVs. In this population of Chinese women with cervical cancer, HPV 52 and 58 were as prevalent as the "high-risk" (for cervical cancer) viruses HPVs 16 and 18.
大量证据已证实人乳头瘤病毒（HPV）感染是人类宫颈癌的病原体。为评估中国女性中HPV与宫颈癌之间的关联，我们检测了居住在中国上海的女性的肿瘤标本。对40名被诊断为鳞状细胞癌（n = 35）或腺癌（n = 5）的女性的活检组织进行PCR检测HPV DNA。肿瘤中存在的HPV类型通过PCR产物与HPV型特异性探针杂交或基于PCR的测序来确定。40份宫颈癌标本中有35份（87.5%）含有HPV DNA。检测到以下分布和类型：7.5%为HPV 16，10%为HPV 18，20%为HPV 16和18，15%为HPV 52，15%为HPV 58，12.5%为HPV 52和58，7.5%为未分类的HPV。在这群患有宫颈癌的中国女性中，HPV 52和58与“高危”（患宫颈癌）病毒HPV 16和18一样普遍。
