Bateman K P, Banoub J H, Thibault P
Department of Chemistry, Dalhousie University, Halifax, NS, Canada.
Electrophoresis. 1996 Dec;17(12):1818-28. doi: 10.1002/elps.1150171205.
The analysis of underivatized oligosaccharides arising from mild acid hydrolysis of endotoxins from Yersinia ruckeri serotype O2 was achieved using on-line capillary zone electrophoresis-electrospray mass spectrometry (CZE-ESMS). This technique provided unparalleled resolution of the different glycans obtained from purified fractions of the native endotoxins or from hydrolysis of lipopolysaccharides from Y. ruckeri. Electrophoretic conditions enabling the separation of anionic and cationic analytes were developed to determine possible sites of heterogeneity on either the core or the O-chain glycans. Structural characterization of underivatized oligosaccharides identified in the ion electropherograms was achieved using tandem mass spectrometry under low-collision energy conditions.
利用在线毛细管区带电泳-电喷雾质谱法（CZE-ESMS）对鲁氏耶尔森菌血清型O2内毒素经温和酸水解产生的未衍生化寡糖进行了分析。该技术为从天然内毒素的纯化级分或鲁氏耶尔森菌脂多糖水解产物中获得的不同聚糖提供了无与伦比的分辨率。开发了能够分离阴离子和阳离子分析物的电泳条件，以确定核心聚糖或O链聚糖上可能的异质性位点。在低碰撞能量条件下，使用串联质谱法对离子电泳图中鉴定出的未衍生化寡糖进行了结构表征。
