Estrada G, Del Rio J A, García-Valero J, López-Tejero M D
Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Spain.
Teratology. 1996 Nov;54(5):245-54. doi: 10.1002/(SICI)1096-9926(199611)54:5<245::AID-TERA5>3.0.CO;2-Z.
The effect of prenatal ethanol exposure on the intestinal maturation of rat fetuses was investigated to understand the nutritional alterations found in the offspring of alcoholic mothers. Female Wistar rats were maintained on solid diet and 25% ethanol solution as drinking fluid during pregnancy, and non-alcoholic isocaloric pregnant mothers were used as controls. At birth, intestines from unsuckled pups were removed for study. The weight and length of the intestine decreased significantly when ethanol was present in utero. Ultrastructural evaluation of the epithelium revealed loss of contact between neighboring enterocytes and abnormal dilation of the cisternae of the Golgi apparatus in ethanol-exposed pups. Further, increased lysosome-like vesiculation and enhanced lysosomal beta-galactosidase activity was observed in these neonates. The total number of absorptive enterocytes in the epithelium was reduced by 30% in ethanol-exposed neonates as compared to controls, due to altered cell growth and death during fetal life. Ethanol in utero stimulated epithelial cell migration which compensated cell loss, as demonstrated by 5'-Bromodeoxyuridine labeling. These findings could have important implications for the assimilation of nutrients and failure to thrive in infants with fetal alcohol syndrome.
为了解酒精性母亲所生后代出现的营养改变情况，研究了产前乙醇暴露对大鼠胎儿肠道成熟的影响。雌性Wistar大鼠在孕期以固体饲料为食，饮用25%乙醇溶液，以非酒精性等热量的怀孕母鼠作为对照。出生时，取出未哺乳幼崽的肠道进行研究。子宫内存在乙醇时，肠道的重量和长度显著下降。对上皮细胞的超微结构评估显示，乙醇暴露幼崽的相邻肠上皮细胞之间失去接触，高尔基体池出现异常扩张。此外，在这些新生儿中观察到类似溶酶体的囊泡化增加以及溶酶体β-半乳糖苷酶活性增强。与对照组相比，乙醇暴露新生儿上皮中吸收性肠上皮细胞总数减少了30%，这是由于胎儿期细胞生长和死亡发生改变所致。子宫内的乙醇刺激上皮细胞迁移，从而补偿细胞损失，5'-溴脱氧尿苷标记证明了这一点。这些发现可能对胎儿酒精综合征婴儿的营养吸收和发育不良具有重要意义。
