Dement'eva E I, Ugarova N N, Cobbold P H
Department of Human Anatomy and Cell Biology, University of Liverpool, United Kingdom.
Biokhimiia. 1996 Jul;61(7):1285-93.
Recombinant firefly luciferase was expressed in E. coli and its properties inside the intact cells were studied. At low concentrations, antibiotic polymyxin B increases permeability of E. coli cell membrane and concentrations of luciferase substrates inside the cells can thus be varied. Effect of intracellular ATP concentrations on intensity of bioluminescence was studied. Recombinant cells expressing the firefly luciferase gene can be used for investigation of substances which influence synthesis and hydrolysis of ATP inside the cells and cell membrane transport.
重组萤火虫荧光素酶在大肠杆菌中表达，并对其在完整细胞内的特性进行了研究。在低浓度下，抗生素多粘菌素B可增加大肠杆菌细胞膜的通透性，从而改变细胞内荧光素酶底物的浓度。研究了细胞内ATP浓度对生物发光强度的影响。表达萤火虫荧光素酶基因的重组细胞可用于研究影响细胞内ATP合成与水解以及细胞膜转运的物质。
